%0 Journal Article
%T Isolation of high quality RNA from Polyporus umbellatus (Pers.) Fries
%A Zhang
%A Yuejin
%A Qin
%A Yali
%A Guo
%A Lijun
%A Zhou
%A Zili
%A Liang
%A Zongsuo
%A Zhang
%A Chenlu
%A Guo
%A Hongbo
%J Electronic Journal of Biotechnology
%D 2012
%I Pontificia Universidad Cat¨®lica de Valpara¨ªso
%X background: the dried sclerotium of medicinal fungus polyporus umbellatus (pers.) fries has many pharmacological functions such as diuretic and anticancer activity, in which high-content polysaccharides may play an important role. however, rna isolation is difficult in filamentous fungi and lacking in p. umbellatus. results: five methods for rna extraction from five strains collected from four provinces were assessed for their ability to recover a high-quality rna applicable for sequence-related amplification polymorphism (srap) pcr and gdp-d-mannose pyrophosphorylase (gmp) gene expression profiles. both a260/a280 and a260/a230 ratios of the best trizol plus + rnaiso-mate for plant tissue method are around 2 with a yield of 1122.00 ¡À 0.21 ng ¦Ìl-1. the trizol method also showed good quality with the yield 469.60 ng ¦Ìl-1. the srap pcr amplified clear and polymorphic bands in all five cdna samples transcribed from rna by using primer me4-em4. gmp gene fragment (1251 bp) was successfully amplified by rt-pcr, suggesting the integrity of isolated rna. conclusion: all these results showed that the total rna isolated by this protocol is of sufficient quality for subsequent molecular applications.
%K chuling
%K medicinal fungus
%K polyporus umbellatus
%K rna isolation.
%U http://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0717-34582012000500010&lng=en&nrm=iso&tlng=en