%0 Journal Article
%T Development of transformation vectors for the production of potentially high oleate transgenic oil palm
%A Masani
%A Mat Yunus Abdul
%A Parveez
%A Ghulam Kadir Ahmad
%J Electronic Journal of Biotechnology
%D 2008
%I Pontificia Universidad Cat¨®lica de Valpara¨ªso
%X the main target of malaysian palm oil board (mpob) genetic engineering programme is to produce high oleate transgenic palms. the availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant dna technology. here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (ubipro)-driven bar gene for selection of transformants on herbicide (basta or bialaphos), and mesocarp-specific promoter (msp1) for expression of the transgenes [antisense palmitoyl-acp-thioesterase (pat) and sense ¦Â-ketoacyl-acp-synthase ii (kasii) and sense ¦Ä9-stearoyl-acp-desaturase (sad)] potentially responsible for high oleate content in oil palm mesocarp. the transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and agrobacterium-based transformation protocols.
%K oleate
%K transformation vectors
%K transgenic oil palm.
%U http://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0717-34582008000300003&lng=en&nrm=iso&tlng=en