%0 Journal Article
%T Universal protocol for generating 100bp size standard for endless usage
%A Natarajan
%A Chandrasekhar
%A Vijayan
%A Neetha N
%A Vaidyan
%A Linda Koshy
%A Mathew
%A Anila
%A Srinivas
%A Lekshmy
%A Banerjee
%A Moinak
%J Electronic Journal of Biotechnology
%D 2008
%I Pontificia Universidad Cat¨®lica de Valpara¨ªso
%X developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. this calls for designing of simple and cost-effective alternatives for the indispensable products like dna molecular weight markers. we demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. in our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. these primers are used at a universal annealing temperature to amplify ten dna fragments of accurate size ranging from 100bp to 1000bp. our pcr-based method can provide size standards for an endless usage.
%K 100bp ladder
%K cost effective
%K dna size standards
%K endless usage.
%U http://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0717-34582008000200015&lng=en&nrm=iso&tlng=en