%0 Journal Article
%T Expression of the antibody 14D9 in Nicotiana tabacum hairy roots
%A Mart¨ªnez
%A Carolina
%A Petruccelli
%A Silvana
%A Giulietti
%A Ana Mar¨ªa
%A Alvarez
%A Mar¨ªa Alejandra
%J Electronic Journal of Biotechnology
%D 2005
%I Pontificia Universidad Cat¨®lica de Valpara¨ªso
%X nicotiana tabacum hairy roots that express the antibody 14d9 were established. the 14d9 antibody yield obtained after 20 days of culture was 5.95 ¦Ìg 14d9ml-1. the addition of the reticulum endoplasmic retention sequence kdel demonstrated a positive effect over the intracellular 14d9 amounts with a yield increase up to 20.82 ¦Ìg ml-1. dmso increased the antibody amount in the biomass from 20.00 to 64.03 ¦Ìg ml-1 while pvp (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14d9 amounts in the culture medium to 25 ¦Ìg and 14 ¦Ìg respectively
%K catalytic antibodies
%K in vitro cultures
%K molecular farming
%K recombinant antibodies
%K tobacco.
%U http://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0717-34582005000200006&lng=en&nrm=iso&tlng=en