%0 Journal Article
%T Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method
%A Pereira Duta
%A Fl¨¢via
%A Pess£¿a de Fran£¿a
%A Francisca
%A de Almeida Lopes
%A L¨¦a Maria
%J Electronic Journal of Biotechnology
%D 2006
%I Pontificia Universidad Cat¨®lica de Valpara¨ªso
%X the combined effects of the processing parameters for exopolysaccharides production by rhizobium sp. was studied using the experimental design and response surface methodology. the experiments were carried out using a fermenter with 20 l capacity, as the reactor. all processing parameters were online monitored. the temperature [(30 ¡À 1)oc] and ph value (7.0 ¡À 0.1) were kept constant throughout the experimental time. as statistical tools, a complete 23 factorial planning with central point and response surface were used to study the interactions among three relevant variables of the fermentation process: calcium carbonate concentration, aeration and agitation. the processing parameters setup for reaching a maximum response for exopolysaccharides production was obtained when applying the highest values for calcium carbonate concentration (1.1 g/l), aeration (1.3 vvm) and agitation (800 rpm). in addition, the combination of these optimum processing parameters yielded yp/s (g/g) = 0.35.
%K exopolysaccharides
%K experimental design
%K response surface method
%K rhizobium sp..
%U http://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0717-34582006000400007&lng=en&nrm=iso&tlng=en