%0 Journal Article
%T Microdi¨¢lisis subcut¨¢nea: una t¨¦cnica simple para monitorizar el ambiente bioqu¨ªmico extracelular. Combinaci¨®n con electroforesis capilar y detecci¨®n mediante fluorescencia inducida por l¨¢ser.
%A P¨¢ez
%A Ximena
%A Mazzei-D¨¢vila
%A Carmen Amalia
%A Hern¨¢ndez
%A Luis
%J Investigaci¨®n Cl¨ªnica
%D 2003
%I Scientific Electronic Library Online
%X abstract. microdialysis is a simple technique that allows monitoring endogenous or exogenous substances in any extracellular compartment. it has many useful experimental and clinical applications. the sampling of the extracellular fluid of the subcutaneous compartment is especially useful for metabolic evaluation in critically ill patients, pharmacokinetic studies and blood glucose monitoring. we built a subcutaneous microdialysis probe, with a cellulose hollow fiber (13,000 molecular weight cut off, 200 ¦Ìm outside diameter) glued to stainless steel tubing (26 ga. outside diameter). it was implanted in the subcutaneous tissue of a critically ill child or anesthetized mice to obtain amino acids patterns by means of capillary electrophoresis with laser induced fluorescence detection (ce-lifd). the probe was also implanted in ambulatory volunteers to monitor glucose. the results confirmed that subcutaneous microdialysis is a very simple, inexpensive and not aggressive method with advantages over repeated venipuncture sampling and endovenous microdialysis sampling. the present report shows that subcutaneous microdialysis with the proper analytical technique can be used to monitor the chemical composition of the interstitial compartment in very different preclinical or clinical conditions.
%K mice
%K human
%K electrokinetic micellar chromatography
%K neutral amino acids
%K glucose
%K pharmacokinetics
%K ambulatory and critically ill patients.
%U http://www.scielo.org.ve/scielo.php?script=sci_abstract&pid=S0535-51332003000300006&lng=en&nrm=iso&tlng=en