%0 Journal Article
%T Diagn¨®stico molecular de candidemia mediante PCR semianidada en pacientes cr¨ªticos
%A Ospina
%A William Pascual
%A Cort¨¦s
%A Jorge Alberto
%J Acta Medica Colombiana
%D 2011
%I Asociacion Colombiana de Medicina Interna
%X objectives: to standardize a molecular test based on semi-nested pcr for diagnosis of candidemia. methods: we developed a pilot study by means of optimizing the extraction protocol and use of ribosomal dna, amplifying the complete its-2 region of candida spp. and by semi-nested pcr reamplifying specific segments of nine clinically relevant candida species. clinical samples of plasma of critically ill patients with microbiologically proven candidemia from a third level hospital in bogot¨¢. another sample of simulated candidemia was used with candida strain form a urine sample. results: we were able to standardize this technique and achieved from clinical samples of plasma a likely sensitivity of 90% and a theoretical specificity of 100%. the detection capability of this test is up to 1 cell per 200 ¦Ìl of plasma. conclusion: a molecular test for detection of candidemia among critically ill patients was standardized (acta med colomb 2011; 36: 135-140).
%K (decs)
%K candidemia/microbiology
%K candida
%K candida/isolation & purification
%K candidemia
%K candidemia/diagnosis
%K candidemia/microbiology
%K molecular diagnostic technology
%K polymerase chain reaction
%K nested pcr.
%U http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0120-24482011000300005&lng=en&nrm=iso&tlng=en