%0 Journal Article
%T Experimental canine model for sentinel lymph node biopsy in the vulva using technetium and patent blue dye
%A Aquino
%A Jos¨¦ Ulcijara
%A Pinheiro
%A Luiz Gonzaga Porto
%A Vasques
%A Paulo Henrique Di¨®genes
%A Rocha
%A Jo£¿o Ivo Xavier
%A Cruz
%A Diego Alves
%A Beserra
%A Hugo Enrique Orsini
%A Cavalcante
%A Raissa Vasconcelos
%J Acta Cirurgica Brasileira
%D 2012
%I Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
%R 10.1590/S0102-86502012000200002
%X purpose: this paper aims to study and define the experimental model of sentinel lymph node biopsy of the vulva in bitches. methods: 0.2 ml of 99mtc phytate was injected intradermally, using a fine gauge insulin needle in the anterior commissure of the vulva. thirty minutes after 99mtc injection, the inguinal mapping was performed using a gamma probe. after this, 0.5 ml of blue dye (bleu patent¨¦ v guerbet 2.5%) was injected in the same place. after 15 minutes, a 3 cm long inguinal incision was made at point maximum uptake followed by careful dissection, guided by visualization of a bluish afferent lymphatic system that points to the sentinel lymph node (sln). results: it was observed that 88% of sln were identified. it wasn¡ät found a significant difference among the presence or not of sentinel lymph node in the sides, which is an indication of a good consistency. it was observed a high (88%) and significant (¦Ö2=12.89 and p=0.0003) intercession between both methods (blue dye and radiation). conclusion: the experimental model adopted is feasible, becoming advantageous in applying the association of patent blue and 99mtc.
%K vulvar neoplasms
%K sentinel lymph node biopsy
%K technetium
%K animal experimentation
%K dogs.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0102-86502012000200002&lng=en&nrm=iso&tlng=en