%0 Journal Article
%T 乙醇–硫酸铵双水相辅助超声波提取枇杷叶黄酮工艺优化及抑菌活性测定
Optimization of Ethanol-Ammonium Sulfate Aqueous Two-Phase Assisted Ultrasound Extraction of Flavonoids from Loquat Leaves and Determination of Their Antibacterial Activity
%A 薛冰
%A 廖礼艳
%A 刘丽娟
%A 陈丹
%A 赵国梅
%A 杨姗
%J Journal of Organic Chemistry Research
%P 195-205
%@ 2330-524X
%D 2025
%I Hans Publishing
%R 10.12677/jocr.2025.132020
%X 枇杷叶中黄酮类物质的提取采用超声波辅助乙醇硫酸铵双水相体系，进行单因素和正交试验。结果发现，枇杷叶黄酮的更优工艺是超声辅助双水相提取。超声功率420 W，超声温度50℃，超声时间40 min，液料比25 mL/g，硫酸铵用量7 g，醇水比0.6为最佳工艺条件，黄酮类在此条件下浸出量为8.01 mg/g。在体外抑菌活性研究中，该提取物对絮状表皮癣菌表现出最强的抑制作用，其最低抑菌浓度和最小杀菌浓度均为0.0029 g/mL。
This study optimized the extraction process of flavonoids from loquat leaves using an ethanol-ammonium sulfate aqueous two-phase system coupled with ultrasound-assisted extraction. Single-factor and orthogonal experiments were conducted to determine the optimal conditions. The results revealed that the superior extraction parameters were as follows: ultrasound power of 420 W, temperature of 50˚C, extraction time of 40 min, liquid-to-material ratio of 25 mL/g, ammonium sulfate dosage of 7 g, and ethanol-to-water ratio of 0.6. Under these conditions, the flavonoid yield reached 8.01 mg/g. In vitro antibacterial activity tests demonstrated that the extract exhibited the strongest inhibitory effect against Trichophyton mentagrophytes, with both the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) being 0.0029 g/mL.
%K 枇杷叶，
%K 黄酮，
%K 超声波，
%K 乙醇–
%K 硫酸铵，
%K 抑菌活性
Loquat Leaf
%K Flavonoids
%K Ultrasound
%K Ethanol-Ammonium Sulfate
%K Antibacterial Activity
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=118435