%0 Journal Article %T Determination of Nifuratel in Human Plasma by Using Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Pharmacokinetic Study %A Nihal Saraner %A Gö %A kç %A e Demiray %A Berrak Gü %A ney %A Onursal Sağ %A lam %J Open Journal of Applied Sciences %P 1486-1494 %@ 2165-3925 %D 2025 %I Scientific Research Publishing %R 10.4236/ojapps.2025.155103 %X In this study, a rapid, straightforward, and sensitive method was developed and validated for the quantification of nifuratel (NIF) in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Plasma samples were processed using liquid-liquid extraction with nifuratel 13C D3 as the internal standard (IS). Chromatographic separation was achieved on a Waters ACQUITY UPLC BEH C18 (1.7 µm, 2.1 × 50 mm) column using a mobile phase consisting of formic acid, water, and acetonitrile at a flow rate of 0.60 mL/min. NIF was identified and quantified via mass spectrometry with electrospray ionization (ESI) in positive ion mode and multiple reaction monitoring (MRM). The method showed linearity in the range of 0.25 - 150 ng/mL for NIF. The developed and validated method was applied to the bioequivalence study in human plasma samples. %K Nifuratel %K Nifuratel 13C D3 %K Human Plasma %K LC-MS/MS %K Bioequivalence %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=142897