%0 Journal Article %T 聚氨酯三维肺癌模型用于盐酸青藤碱对肺癌细胞侵袭转移蛋白分泌影响的研究
Establishment of Three-Dimensional Polyurethane Model of Lung Cancer and Effect of Sinomenine Hydrochloride on Invasion and Metastasis Protein Secretion of Lung Cancer Cells %A 王晗 %A 黄逸伦 %A 朱莲 %A 张文元 %A 唐靓 %J World Journal of Cancer Research %P 108-114 %@ 2164-9057 %D 2025 %I Hans Publishing %R 10.12677/wjcr.2025.153014 %X 目的：探讨通过三维(3D)聚氨酯泡沫支架构建肺癌模型，并用于盐酸青藤碱影响肺癌A549细胞分泌侵袭转移蛋白的可行性。方法：采用聚氨酯泡沫支架构建3D肺癌模型，进行不同剂量盐酸青藤碱对肺癌A549细胞作用实验，ELISA法测定A549细胞培养上清中基质金属蛋白酶(MMP-2, MMP-9)和上皮间质转化(EMT)标记物E-cadherin、N-cadherin、Vimentin的表达，观察迁移及侵袭相关蛋白分泌情况。结果：盐酸青藤碱可有效抑制A549细胞培养上清液中MMP-2、MMP-9的表达，以及有效抑制EMT，表现为下调N-cadherin、Vimentin蛋白表达，上调E-cadherin蛋白表达。随着盐酸青藤碱浓度的增加，抑制增加，呈剂量依赖性。对3D培养的抑制显著低于2D培养。结论：与2D培养相比，本实验中建立的基于聚氨酯的3D肺癌模型可能更好地模拟了体内病理生理状况，更具预测及分析药物能力。
Objective: To investigate the feasibility of constructing a lung cancer model with three-dimensional (3D) polyurethane foam scaffold and using sinomenine hydrochloride to influence the secretion of invasion and metastasis protein in A549 cells of lung cancer. Methods: A three-dimensional lung cancer model was constructed with polyurethane foam scaffold, and the effects of sinomenine hydrochloride on A549 cells were tested. The expression of matrix metalloproteinases (MMP-2, MMP-9) and epithelial-mesenchymal transition (EMT) markers E-cadherin, N-cadherin and Vimentin in the supernatant of A549 cell culture was determined by ELISA, and the secretion of migration and invasion related proteins was observed. Results: Sinomenine hydrochloride could effectively inhibit the expression of MMP-2 and MMP-9 in the supernatant of A549 cell culture, and effectively inhibit EMT, such as down-regulating the expression of N-cadherin and Vimentin, and up-regulating the expression of E-cadherin. With the increase of sinomenine hydrochloride concentration, the inhibition increased in a dose-dependent manner. The inhibition of 3D culture was significantly lower than that of 2D culture. Conclusion: Compared with 2D culture, the 3D lung cancer model based on polyurethane established in this study may better simulate the pathophysiology in vivo, and has more ability to predict and analyze drugs. %K 聚氨酯泡沫， %K 三维肺癌模型， %K 盐酸青藤碱， %K 迁移侵袭蛋白， %K 基质金属蛋白酶， %K 上皮间质转化
Polyurethane Foam %K 3D Lung Cancer Model %K Sinomenine Hydrochloride %K Migration and Invasion Protein %K Matrix Metalloproteinases %K Epithelial-Mesenchymal Transition %U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=113732