%0 Journal Article %T Progranulin通过调控巨噬细胞极化对烟曲霉菌性角膜炎发挥保护作用的研究
Research on a Protective Role of Progranulin in Aspergillus fumigatus Keratitis by Modulating Macrophage Polarization %A 贺铜 %A 段慧瑾 %A 刘婧仪 %A 亓平丽 %J Advances in Clinical Medicine %P 3024-3032 %@ 2161-8720 %D 2025 %I Hans Publishing %R 10.12677/acm.2025.1541268 %X 目的：探究progranulin (颗粒蛋白前体，PGRN)在烟曲霉菌角膜炎中发挥抗炎作用的调控机制。方法：建立烟曲霉菌小鼠角膜炎模型，前节照相和临床评分分析PGRN对小鼠角膜病变程度的影响；HE染色检测小鼠角膜组织中炎症细胞的数量；利用CCK-8实验，检测不同浓度梯度的PGRN对小鼠巨噬细胞(RAW264.7)的安全性；实时定量聚合酶链反应(RT-PCR)检测烟曲霉菌感染的细胞中，PGRN对诱导型一氧化氮合酶(iNOS)、趋化因子(CCL2)、精氨酸酶1 (Arg-1) mRNA表达的影响；采用流式细胞术检测PGRN对M2/M1比值的影响。结果：PGRN减轻小鼠烟曲霉菌角膜炎病变程度；PGRN减轻小鼠角膜组织中炎症细胞的浸润；PGRN对RAW264.7细胞无毒；10 ng/ml PGRN处理RAW264.7后显著降低iNOS、CCL2的mRNA表达水平，升高Arg-1的mRNA水平，且PGRN增加了M2/M1的比值。结论：PGRN可能通过调节巨噬细胞极化在烟曲霉菌角膜炎中发挥抗炎作用。
Objective: To investigate the regulatory mechanism by which progranulin (PGRN) exerts anti-inflammatory effects in Aspergillus fumigatus keratitis. Methods: A mouse keratitis model of A. fumigatus was established, and the effects of PGRN on the degree of corneal lesions in mice were analyzed by anterior segment photography and clinical scoring; HE staining was used to detect the number of inflammatory cells in the corneal tissues of mice; using the CCK-8 assay, the safety of PGRN at different concentration gradients on mouse macrophages (RAW264.7) was examined; and real-time quantitative polymerase chain reaction (RT-PCR) detected the effects of PGRN on the mRNA expression of inducible nitric oxide synthase (iNOS), chemokine (CCL2), and arginase 1 (Arg-1) in Aspergillus fumigatus-infected cells; and the effects of PGRN on the M2/M1 ratio were detected by flow cytometry. Results: PGRN attenuated the extent of mouse Aspergillus fumigatus keratitis lesions; PGRN reduced the infiltration of inflammatory cells in mouse corneal tissues; PGRN was nontoxic to RAW264.7 cells; 10 ng/ml PGRN treatment of RAW264.7 significantly decreased the mRNA expression levels of iNOS and CCL2, and markedly elevated the mRNA level of Arg-1, and PGRN increased the M2/M1 ratio. Conclusion: PGRN may exert anti-inflammatory effects in Aspergillus fumigatus keratitis by regulating macrophage polarization. %K PGRN， %K 角膜炎， %K 烟曲霉菌， %K 巨噬细胞极化， %K 抗炎
PGRN %K Keratitis %K Aspergillus fumigatus %K Macrophage Polarization %K Anti-Inflammation %U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=112854