%0 Journal Article
%T 基于网络药理学和分子对接探究马铃薯调节高血压的作用机制
Exploring the Mechanism of Potato Regulating Hypertension Based on Network Pharmacology and Molecular Docking
%A 黄金连
%A 张彤
%A 叶亦心
%A 周立红
%J Bioprocess
%P 67-79
%@ 2164-5582
%D 2025
%I Hans Publishing
%R 10.12677/bp.2025.151010
%X 目的:探究马铃薯影响高血压的活性成分和可能作用机制。方法:在知网、万方、维普、TCMSP、PubChem、HERB、DisGeNET等数据库检索马铃薯的潜在活性成分、靶点以及高血压相关靶点。将交集靶点导入STRING和Cytoscape3.10.3软件中进行分析,并利用CytoHCA、CytoHubba和MCODE插件挖掘马铃薯影响高血压的核心基因。应用DAVID数据库进行GO和KEGG富集剖析,利用Auto Dock Tools-1.5.6和PyMOL软件进行分子对接和可视化分析。结果:得到马铃薯活性成分15个,化合物预测靶点578个,高血压靶点2687个,马铃薯活性成分和高血压交集靶点299个。拓扑学分析后得到9个马铃薯影响高血压的核心靶点蛋白。GO和KEGG富集分析显示涉及多个生物过程、细胞组分和分子功能。关键信号通路包含GAPDH、MTOR、HIF1A、BCL2、AKT1和STAT3等。核心活性成分包括天竺葵色素(Pelargonidin)、矢车菊色素(Cyanidin)、芍药色素(Peonidin)、飞燕草色素(Delphinidin)和牵牛花色素(Petunidin)。分子对接结果提示活性成分与作用靶点能稳定结合,特别是GAPDH、MTOR和HIF1A与5种活性成分的结合能都很低,提示马铃薯活性成分有可能通过调节相关信号通路影响高血压。结论:本研究通过网络药理学分析和分子对接发现了马铃薯影响高血压的关键活性成分和核心作用靶点,花色苷对GAPDH和mTOR等靶点的调节值得深入研究。
Objective: To explore the active ingredients and possible mechanisms of potatoes regulating hypertension. Method: Retrieve potential active ingredients, targets, and hypertension related targets of potatoes from databases such as CNKI, Wanfang, VIP, TCMSP, PubChem, HERB, and DisGeNET. Import the intersection targets into STRING and Cytoscape3.10.3 software for analysis, and use CytoHCA, CytoHubba, and MCODE plugins to explore the core genes for potato affecting hypertension. Perform GO and KEGG enrichment analysis using DAVID database, and perform molecular docking and visualization analysis using Auto Dock Tools-1.5.6 and PyMOL software. Results: 15 active ingredients were obtained for potatoes, 578 predicted targets for bioactive compounds, 2687 targets for hypertension, and 299 intersecting targets between potato active ingredients and hypertension. After topological analysis, 9 core target proteins affecting hypertension in potatoes were identified. GO and KEGG enrichment analysis showed involvement in multiple biological processes, cellular components, and molecular functions. The key signaling pathways include GAPDH, MTOR, HIF1A, BCL2, AKT1, and STAT3. The core active ingredients include Pelargonidin, Cyanidin, Peonidin, Delphinidin, and Petunidin. The molecular docking results indicate that the active ingredients can stably bind to the target, especially GAPDH, MTOR, and HIF1A, which have low binding energies with the five active ingredients. This suggests that potato active ingredients may affect hypertension by regulating related signaling pathways. Conclusion: Through network pharmacology analysis and molecular docking, this study discovered the key active ingredients and core targets of potato affecting hypertension. The regulation of anthocyanins on targets such as GAPDH and mTOR deserves further investigation.
%K 马铃薯,
%K 高血压,
%K 网络药理学,
%K 作用机制,
%K 分子对接
Potato
%K Hypertension
%K Network Pharmacology
%K Mechanism of Action
%K Molecular Docking
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=109891