%0 Journal Article %T Multifaceted Analysis of Methylenetetrahydrofolate Reductase Gene Polymorphism in Reproductive-Age Women %A Yumei Ma %A Xiaojuan Jiang %A Xiaoyu Jiang %A Yuanrong Shao %J Natural Science %P 13-19 %@ 2150-4105 %D 2025 %I Scientific Research Publishing %R 10.4236/ns.2025.173003 %X Objective: This study aims to investigate the distribution of methylenetetrahydrofolate reductase (MTHFR) gene 677C>T polymorphism in this region, to guide reproductive-age women in personalized folic acid supplementation. Methods: A total of 1079 reproductive-age women who underwent prenatal examinations at our hospital from July 2020 to July 2024 were selected for the study, and their basic data were collected. The MTHFR gene 677C>T polymorphism was detected using the polymerase chain reaction-fluorescent probe method. Statistical methods were employed to analyze the impact of ethnicity, age groups, and regional differences on MTHFR gene polymorphism and genotype frequency. Results: Among the 1079 subjects, the detection of MTHFR gene 677C>T polymorphism revealed that the 677CC genotype accounted for 62.0%, the 677CT genotype for 33.8%, and the 677TT genotype for 4.2%. There was no significant difference in genotype distribution between different ethnic groups (Zhuang and Han) (P > 0.05), nor were there significant differences observed in different age groups (<20 years, 20 - 30 years, and >30 years) (P > 0.05). However, a significant difference in genotype distribution was found based on different regions (P < 0.01). Conclusion: This study found no significant difference in MTHFR gene 677C>T polymorphism between Zhuang and Han women of reproductive age, but significant regional differences exist. Formulating a scientifically sound folic acid supplementation plan based on the regional distribution characteristics of MTHFR gene polymorphism is crucial for optimizing pregnancy health management. %K Methylenetetrahydrofolate Reductase (MTHFR) %K Gene Polymorphism %K 677C> %K T %K Reproductive-Age Women %K Folic Acid Supplementation %K Ethnic Differences %K Regional Differences %K Pregnancy Health %K Prenatal Examination %K Folic Acid Utilization Risk %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=140880