%0 Journal Article
%T Recombination and Mutation of a New Chinese Strain of HP-PRRSV SCMY2023
%A Xianchang Zhu
%A Wenhui Liu
%A Yang Xia
%A Zhaoxia Yang
%A Qian Lei
%A Yi Zhang
%A Bo Zhang
%A Jida Li
%J Journal of Biosciences and Medicines
%P 338-358
%@ 2327-509X
%D 2025
%I Scientific Research Publishing
%R 10.4236/jbm.2025.131027
%X In this study, RT-PCR was performed on lung tissue samples from sick pigs in a suspected outbreak of porcine reproductive and respiratory syndrome (PRRS) at a pig farm in Mianyang City, Sichuan Province, China. Positive samples were inoculated into Marc-145 cells to observe lesions. The Marc-145 cells with cytopathic lesions were identified by indirect immunofluorescence. The whole genome sequences of the isolated and purified strains were amplified by RT-PCR and analyzed for homology and genetic evolution. A strain of porcine reproductive and respiratory syndrome virus (PRRSV), named SCMY2023 (GenBank No. PQ179742), was successfully isolated. SCMY2023 has a genome length of 15,321 base pairs (without a poly A tail). Nucleotide and amino acid homology analyses suggest that this strain belongs to Lineage 8, a variant of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) prevalent in China. Recombination and genetic evolution analyses indicate that this isolate is a PRRSV variant that recombined with HuN-ZZ (Lineage 8, 98.79% homology) on the backbone of the SCSN2020 strain (Lineage 8, 99.35% homology) in the recombination region from 4407 to 13,107 nucleotides (ORF1a to ORF3). In-depth study of the genetic recombination of this isolate can provide a reference for the prevention and control of PRRS.
%K PRRSV
%K Isolation and Identification
%K Recombinant Virus
%K Prevention and Control Strategy
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=140303