%0 Journal Article %T 急性低盐对黄鳍金枪鱼(Thunnus albacares)鳃、肝、肌肉组织抗氧化的影响
Antioxidant Effects of Gill, Liver, and Muscle Tissues of Yellowfin Tuna (Thunnus albacares) under Acute Low-Salt Conditions %A 张宁璐 %A 周胜杰 %A 马振华 %A 孙金辉 %J Open Journal of Fisheries Research %P 243-254 %@ 2373-1451 %D 2024 %I Hans Publishing %R 10.12677/ojfr.2024.114028 %X 为探究急性低盐对黄鳍金枪鱼幼鱼抗氧化指标的影响，将72尾体长(28.97 ± 2.17) cm，体质量(646.52 ± 66.32) g，随机放入29‰和34‰ (对照组)装有循环过滤海水系统的5000 L玻璃钢水箱中胁迫48 h，分别于胁迫期(0, 6, 24, 48 h)进行取样检测各项指标。实验结果显示：胁迫48 h后LPO、T-AOC、LDH、CAT活性均与对照组无显著差异性(P > 0.05)。LPO酶活力在鳃、肝脏组织中24 h内不断上升并影响显著(P < 0.0 5)，肌肉组织无显著影响(P > 0.05)；T-AOC能力则在鳃、肝脏组织中受到抑制(P < 0.0 5)，肌肉组织中T-AOC能力先升后降的变化趋势，胁迫结束均恢复至对照组水平；LDH酶活性各组织活力均在24 h显著低于对照组(P < 0.05)，至胁迫结束可以恢复至对照组水平；CAT酶活力在鳃组织中影响最为显著(P < 0.05)，肝脏组织总体呈现升高的变化趋势，肌肉组织酶活力总体呈现先降后升的变化趋势，且显著低于对照组(P < 0.05)。实验结果表明，急性低盐对黄鳍金枪鱼幼鱼抗氧化能力产生了显著影响，但经过48 h胁迫后基本恢复至对照组水平，证明黄鳍金枪鱼幼鱼在面对急性低盐条件下，具有良好的抗氧化调节能力。研究结果有助于进一步了解黄鳍金枪鱼在养殖过程中面对急性降水条件下的抗氧化功能的状况，从而提高人工养殖条件下提供合理的数据参考。
In order to investigate the effect of acute low salt on the antioxidant indexes of juvenile yellowfin tuna, 72 fish with body length (28.97 ± 2.17) cm and body mass (646.52 ± 66.32) g were randomly put into a 5000 L glass fiber reinforced plastic tank equipped with circulating and filtering seawater system of 29‰ and 34‰ (control group) for 48 h. Samples were taken to test the indexes in the duress period (0, 6, 24, 48 h), respectively. The samples were taken during the stress period (0, 6, 24 and 48 h). The results showed that the activities of LPO, T-AOC, LDH, and CAT were not significantly different from those of the control group after 48 h. The enzyme activity of LPO increased in gill and liver tissues within 24 h (P < 0.05), but muscle tissues were not significantly affected (P < 0.05), while the capacity of T-AOC was inhibited in gill and liver tissues (P < 0.05), and muscle tissues (P < 0.05). T-AOC capacity was inhibited in gill and liver tissues (P < 0.05), and T-AOC capacity in muscle tissues showed a trend of increasing and then decreasing, and then recovered to the control level at the end of the stress; LDH enzyme activity in all tissues was significantly lower than that of the control group at 24 h (P < 0.05), and then recovered to the control level at the end of the stress; CAT enzyme activity had the most significant effect in gill tissues (P < 0.05), and showed an overall increase in liver tissues (P < 0.05). The enzyme activity of muscle tissue showed a general trend of decreasing and then increasing, and was significantly lower than that of the control group (P < 0.05). The experimental %K 黄鳍金枪鱼， %K 鳃， %K 肝脏， %K 肌肉， %K 抗氧化功能
Thunnus albacares %K Gill %K Liver %K Muscle %K Antioxidant Function %U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=103555