%0 Journal Article
%T Preparative Fractionation of Feruloyl Oligosaccharides Produced by Combinatorial Enzyme Digestion of Arabinoxylan
%A Dominic W. S. Wong
%A Sarah Batt
%A William John Orts
%J Advances in Enzyme Research
%P 35-43
%@ 2328-4854
%D 2024
%I Scientific Research Publishing
%R 10.4236/aer.2024.123003
%X Pretreated wheat insoluble arabinoxylan was converted to oligosaccharides of structural variants using combinatorial enzyme approach. The digestive products were separated by preparative scale chromatographic Amberlite XAD-2 column. Fractions containing feruloyl oligosaccharides (FOS) were isolated, pooled, freeze-dried, and demonstrated to possess antimicrobial activity. The FOS suppressed cell growth of the test organism ATCC 8739 <i>E. coli</i> with a MIC value of 0.028% (w/v, 35&#730;C, 24 hr). The antimicrobial action was observed exceeding 72 hr of culture incubation. The FOS product could be a useful source of prebiotics or preservatives. The present results further confirm the science and application of the concept of combinatorial enzyme technique.
%K Format
%K Wheat Insoluble Arabinoxylan
%K Feruloyl Oligosaccharide
%K Combinatorial Enzyme Digestion
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=137265