%0 Journal Article
%T 直接巢式PCR结合测序检测5-HT1A基因C-1019G多态性的方法的建立<br>Establishment of Direct Nested PCR Combined with Sequencing for Detection of C-1019G Polymorphism in 5-HT1A Gene
%A 王川均
%A 杨玉娟
%A 何震宇
%A 潘伟
%J Hans Journal of Biomedicine
%P 645-651
%@ 2161-8984
%D 2024
%I Hans Publishing
%R 10.12677/hjbm.2024.144070
%X 目的&#65306;5-HT1A基因C-1019G多态性与多种神经精神疾病如抑郁症、精神分裂症、焦虑症等的发生风险、症状严重程度及治疗效果存在关联。本研究旨在建立一种直接巢式PCR结合测序检测该位点的方法。方法&#65306;以口腔上皮细胞粗处理物为材料&#65292;通过直接巢式PCR扩增包含5-HT1A基因C-1019G位点的靶片段&#65292;PCR产物经桑格测序鉴定基因型。结果&#65306;所检样本均能扩增出预期大小的PCR产物&#65292;测序峰图清晰。结论&#65306;成功建立了一种直接巢式PCR结合测序鉴定5-HT1A基因型的方法&#65292;有良好的应用前景。<br>Objective: The C-1019G polymorphism of the 5-HT1A gene has been associated with the risk of occurrence, symptom severity, and treatment outcome of a variety of neuropsychiatric disorders, such as depression, schizophrenia, and anxiety disorders. The aim of this study was to establish a direct nested PCR combined with sequencing to detect this locus. Methods: The target fragment containing the C-1019G locus of the 5-HT1A gene was amplified by direct nested PCR using crude processed oral epithelial cells, and the PCR product was genotyped by Sanger sequencing. Results: PCR products of expected size were amplified from all the samples tested, and the sequencing peaks were clear. Conclusion: A direct nested PCR combined with sequencing method was successfully established to identify the genotypes of 5-HT1A gene, which has good prospects for application.
%K 5-HT1A基因&#65292
%K C-1019G多态性&#65292
%K 直接巢式PCR&#65292
%K 测序<br>5-HT1A Gene
%K C-1019G Polymorphism
%K Direct Nested PCR
%K Sequencing
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=98954