%0 Journal Article
%T Genetic Enhancement of Indigenous Cowpea with Gamma-Ray Induced Trait Variation
%A Mathurin Tiergnin Dabir&#233
%A Minimassom Philippe Niki&#233
%A ma
%A Djibril Yonli
%A Safiatou Sanna
%A Wossoguim Josu&#233
%A Gouba
%A Si&#233
%A bou Pal&#233
%A Hamidou Traor&#233
%A Joseph T. B. Batieno
%A Varra Prasad
%A Zacharia Stewart
%A Jan B. Middendorf
%A Abhishek Rathore
%A Anupama J. Hingane
%J American Journal of Plant Sciences
%P 651-676
%@ 2158-2750
%D 2024
%I Scientific Research Publishing
%R 10.4236/ajps.2024.158044
%X Production of cowpea (<i>Vigna unguiculata</i> (L.) Walp.), a staple legume crop in Sub-Saharan Africa, faces challenges due to biotic and abiotic constraints. Induced mutagenesis was deployed to create genetic variation in two cowpea varieties (KVX396-4-5-2D and Moussa local). The radio-sensitivity tests led to determe the lethal dose 50 (LD<sub>50</sub>) corresponding to 230 Gy and 220 Gy for KVX396-4-5-2D and Moussa local varieties, respectively. Dried seeds (M0) of each variety were gamma-ray irradiated with LD<sub>50</sub> &#8722; 50, LD<sub>50</sub> and LD<sub>50</sub> + 50. M1 seeds were advanced to generate M2, M3 and M4 mutants using the single-seed-descent method. M4 mutant lines were evaluated in rain-fed conditions using a randomized complete block design to assess phenotypic differences. Data on seven qualitative and eleven quantitative traits were collected. The results indicated that the mutation induced variability in three qualitative traits: in KVX 396-4-5-2D mutant lines, with flower and seed color frequencies at 2.61% and 0.56% respectively, and pod dehiscence at a frequency of 0.24%. While in Moussa local mutants, a pod color changed at a frequency of 17%. ANOVA results revealed significant differences between mutants of both varieties for all quantitative traits, including photosynthetic parameters. Positive correlations were observed between leaf diameter and 100-seed weight, and between branch number and 100-seed weight. Hierarchical clustering revealed three clusters among KVX 396-4-5-2D mutants and six clusters among Moussa local mutants. Early maturity and high foliage were induced traits in Cluster 3 of KVX 396-4-5-2D mutants while high hundred-seed weight was induced in Cluster 6 of Moussa local mutants.
%K Cowpea
%K Mutagenesis
%K Gamma Rays
%K Radio-Sensitivity
%K Phenotyping
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=135571