%0 Journal Article
%T Magnetic Field Emulations of Small Inhibitor RNA: Effects on Implanted GL261 Tumors in C57BL/6 Immune Competent Mice
%A Xavier A. Figueroa
%A Gabriel Vogeli
%A B. Michael Butters
%J Open Journal of Biophysics
%P 339-354
%@ 2164-5396
%D 2024
%I Scientific Research Publishing
%R 10.4236/ojbiphy.2024.144013
%X EMulate Therapeutics has developed a system for emulating the effects of solvated molecules via their magnetic field recordings. Recordings of magnetic field emissions of select small inhibitor RNAs (siRNAs; murine targeting CTLA-4 and murine targeting PD-1) were tested on C57Bl/6 mice implanted subcutaneously with the GL261 murine tumor cell line. A signal composed of concatenated recordings of siRNA molecules targeting the murine CTLA-4 and PD-1 receptors (labeled A2) was used in immune competent C57Bl/6 mice. The mice were flank implanted with the murine glioblastoma cell line GL261. Mice were exposed to the signal continuously (24 hours a day) until tumor volumes reached the designated volume limit. Tumors were excised and analyzed via PAGE/Western blot for the expression of CTLA-4, PD-1, Ki67, Caspase 3, CD4 and CD8. Terminal blood draws were used for CBCs. We report the down regulation of the checkpoint inhibitors CTLA-4 in the exposed mice. Significant tumor volume reduction was observed in mice exposed to the siRNA signal compared to control mice; no adverse events were recorded. Cell blood counts (CBC) and protein expression patterns were observed to correlate with the expected function of protein expression inhibition of the targets.
%K Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4)
%K Programmed Cell Death Protein 1 (PD-1)
%K Electromagnetic Field
%K Emulation
%K Cancer
%K Tumor
%K Murine
%K Glioblastoma Multiforme (GBM)
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=135320