%0 Journal Article
%T 续断及其配方颗粒的UPLC指纹图谱研究<br>UPLC Fingerprinting Study of <i>Dipsacus asper</i> Wall. and Its Formulated Granules
%A 许扬
%A 王祥培
%A 吴红梅
%J Pharmacy Information
%P 369-378
%@ 2160-4452
%D 2024
%I Hans Publishing
%R 10.12677/pi.2024.134043
%X 目的：建立续断药材及配方颗粒的UPLC指纹图谱，为续断配方颗粒质量控制提供有效方法。方法：采用UPLC同时测定10批续断药材和10批配方颗粒，制定指纹图谱。色谱柱为Agilent ZORBAX Eclipse Plus C<sub>18</sub> (2.1 mm × 100 mm, 1.8 μm)；流速0.2 mL·min<sup>？</sup><sup>1</sup>；检测波长212 nm；柱温30℃；进样量1 μL；流动相：0.1%磷酸水(A)-乙腈(C)二元梯度洗脱。结果：10批续断药材有25个共有色谱峰，10批续断配方颗粒有32个共有色谱峰，10批续断药材与10批配方颗粒确定25个共有峰，各峰之间分离度较高。10批续断药材和10批配方颗粒的相似度均大于0.8，10批配方颗粒的相似度大于0.9。结论：建立的续断药材和配方颗粒指纹图谱稳定可靠，药材与配方颗粒的色谱峰个数有较大的差异，说明药材经提取、浓缩、制粒等制备工艺后，化学成分有所变化，存在差异。<br />
Objective: To establish the UPLC fingerprints of <i>Dipsacus asper</i> W. and formulated granules, and to provide an effective method for the quality control of the formulated granules of <i>Dipsacus asper</i> W. Methods: UPLC was used to simultaneously determine 10 batches of <i>Dipsacus asper</i> W. and 10 batches of the formulated granules of <i>Dipsacus asper W</i>. and to develop the fingerprint profiles. The chromatographic column was Agilent ZORBAX Eclipse plus C18 (2.1 mm × 100 mm, 1.8 <i>μ</i>m); the flow rate was 0.2 mL？min<sup>？1</sup>; the detection wavelength was 212 nm; the column temperature was 30？C; the injection volume was 1 <i>μ</i>L; and the mobile phase was a binary gradient elution of 0.1% phosphoric acid in water (A)-acetonitrile (C). Result: There were 25 common chromatographic peaks in 10 batches of <i>Dipsacus asper </i>W., 32 common chromatographic peaks in 10 batches of Formula granules, and 25 common peaks were determined between 10 batches of <i>Di</i><i>p</i><i>sacus asper </i>W. and 10 batches of formula particles, with high separation between each peak. The similarity between 10 batches of <i>Dipsacus asper </i>W. and 10 batches of formula particles is greater than 0.8, and the similarity between 10 batches of formula particles is greater than 0.9. Conclusion: The fingerprints of <i>Dipsacus asper </i>W. and Formula granules were stable and reliable and the number of chromatographic peaks of the herbs and the formulated particles had a large difference, indicating that the chemical compositions of the herbs had changed and differed after the preparation process of extraction, concentration and granulation.
%K 续断，配方颗粒，UPLC指纹图谱<br><i>Dipsacus asper</i> Wall.
%K Formulated Granules
%K UPLC Fingerprinting
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=91642