%0 Journal Article
%T 贵州黎平地区沼水蛙的分子鉴定<br>Molecular Identification of a <i>Sylvirana </i><i>guentheri</i> Population Distributed in Liping County, Guizhou Province in China
%A 冯权江
%A 李明
%A 汪莎莎
%A 何婉琴
%A 符静
%A 熊荣川
%J Open Journal of Nature Science
%P 551-560
%@ 2330-1732
%D 2024
%I Hans Publishing
%R 10.12677/ojns.2024.123064
%X 于2015年8月在贵州省黔东南州黎平县德凤镇南泉山采集到2个蛙类标本，经初步形态鉴定为沼水蛙(<i>Sylvirana guentheri</i>)，但之前并没有该物种在黎平县分布的文献记载，因此有必要使用分子手段对其分类地位进行准确界定。本文对采自贵州黎平县的沼水蛙标本进行DNA提取，并克隆其线粒体16S rRNA基因片段，使用分子系统发育学研究方法对其物种进行初步的分子鉴定。结果表明，黎平采蛙类样本16S rRNA基因序列与31条沼水蛙同源序列聚为一个支持率较高的单系，支系内序列间平均遗传距离为0.0056，且对应序列单倍型均在同一个单倍型网络内。自测序列与沼水蛙近模式产地(福建福州)一标本的16S rRNA基因序列遗传距离较小。因此，初步判断该标本属沼水蛙(<i>Sylvirana guentheri</i>)。初步的种群中性检验分析表明，沼水蛙种群最近可能发生过种群扩张。另外，本文探讨了较短的同源序列对相关系统发育分析结果的影响。<br />
In August 2015, two frogspecimens were collected from Nanquan Mountain of Defeng Township, Liping County, Qiandongnan Prefecture in Guizhou Province. They were identified as <i>Sylvirana guentheri</i><i> </i>by preliminary morphological identification. However, it was not documented the distribution of this species in Liping County, so it is necessary to use molecular means to accurately define their taxonomic status. In this paper, DNA extraction and cloning of mitochondrial 16S rRNA gene fragment of <i>Sylvirana guentheri</i><i> </i>from Liping County, Guizhou Province were carried out. The preliminary molecular identification of this population was carried out by using the research method of molecular phylogeny. The results showed that the 16S rRNA gene sequences of Liping frogs and the homologous sequences of 31 <i>Sylvirana guentheri</i><i> </i>frogs were clustered into a monophyletic clade with high support rates. The average genetic distance between sequences in the branch was 0.0056, and the corresponding haplotypes were all in the same haplotype network. The genetic distance between the 16S rRNA gene sequence of Liping frogs and the homologous sequence of a sample collected near type localitiy (Fuzhou, Fujian) was 0.008. Therefore, it is preliminarily determined that this specimen belongs to <i>Sylvirana guentheri</i>. The neutrality tests of <i>S. guentheri</i> population showed that the population may have expanded recently in history. In addition, the influence of shorter homologous sequences on the results of related phylogenetic analysis was discussed.
%K 沼水蛙，16S rRNA，分子鉴定，黎平，短序列<br><i>Sylvirana guentheri</i>
%K 16S rRNA
%K Molecular Identification
%K Liping
%K Shorter Homologous Sequences
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=87265