%0 Journal Article
%T Phenotypic Characterization of Extended Spectrum Beta-Lactamase, Class C Cephalosporinase and Carbapenemase-Producing &lt;i&gt;Klebsiella&lt;/i&gt; Species Isolated from Patients Consulted at Four Yaounde-Based Hospitals
%A Emilia Enjema Lyonga Mbamyah
%A Mangum Patience Kumcho
%A Michel Toukam
%A Dieudonn&#233
%A Sedena
%A Florence Anjabie Enyeji
%A Aime-Caesar Teukam
%A Modestine Djuissi
%A Martha Tongo Mesembe
%A George Mondinde Ikomey
%A Agnes Bedie Eyoh
%A Hortense Kamga Gonsu
%J Open Journal of Medical Microbiology
%P 105-119
%@ 2165-3380
%D 2024
%I Scientific Research Publishing
%R 10.4236/ojmm.2024.142009
%X &lt;b&gt;Background:&lt;/b&gt; &lt;i&gt;Klebsiella&lt;/i&gt; spp. are bacteria of medical importance for their role in opportunistic infections which are often difficult to treat because of resistance to one or several antimicrobials. The aim of this study was to determine antimicrobial resistance due to Extended Spectrum Beta-lactamase (ESBL), Class C cephalosporinase (AmpC) and carbapenemase enzymes in &lt;i&gt;Klebsiella&lt;/i&gt; &lt;i&gt;spp.&lt;/i&gt; isolated from patients consulted at four hospitals. &lt;b&gt;Methodology:&lt;/b&gt; The study was cross-sectional and descriptive. A total of 4190 non-repetitive patients&amp;#8217; specimens from 13 types of clinical specimens were analysed from February to November 2020. Two hundred and twenty-five (225) &lt;i&gt;Klebsiella&lt;/i&gt; &lt;i&gt;spp&lt;/i&gt; isolates were identified using API 20E and antimicrobial susceptibility testing done according to the Kirby Bauer disc diffusion method. ESBL and AmpC phenotypes were determined by the combination disc method and carbapenemases by double disc synergy method, referenced by EUCAST guidelines for the resistance testing. &lt;b&gt;Results:&lt;/b&gt; The frequency of the species was &lt;i&gt;Klebsiella&lt;/i&gt; &lt;i&gt;pneumoniae&lt;/i&gt; (69%, 155/255), &lt;i&gt;K.&lt;/i&gt; &lt;i&gt;oxytoca&lt;/i&gt; (14%, 31/255), &lt;i&gt;K.&lt;/i&gt; &lt;i&gt;ozaenae&lt;/i&gt; (12%, 27/225) and &lt;i&gt;K.&lt;/i&gt; &lt;i&gt;rhinoscleromatis&lt;/i&gt; (5%, 11/225). Isolates were most resistant to sulphomethoxazole trimethoprim (84%, 189/225), cepaholosporins (80%, 180/225), and least resistant to carbapenems (10.7%, 24/225). Two &lt;i&gt;K.&lt;/i&gt; &lt;i&gt;oxytoca&lt;/i&gt; and one &lt;i&gt;K.&lt;/i&gt; &lt;i&gt;pneumoniae&lt;/i&gt; were resistant to all antibiotics tested. &lt;i&gt;Kle&lt;/i&gt;&lt;i&gt;b&lt;/i&gt;&lt;i&gt;siella&lt;/i&gt; &lt;i&gt;pneumoniae&lt;/i&gt; had the most multidrug resistant isolates (59.4%, 134/225). Most isolates (83.6%, 188/225) expressed at least one enzyme, while 63.6% (143/225) of the isolates expressed at least two enzymes. Some isolates were ESBL (71.6%, 161/225), carbapenemase (10.7%, 24/225) and AmpC (6.6%, 15/225) producers. Three carbapenemases (&lt;i&gt;Klebsiella&lt;/i&gt; &lt;i&gt;pneumoniae&lt;/i&gt; carbapenemase-KPC, Metallo-Beta Lactamase-MBL and OXA-48) were detected. &lt;b&gt;Co&lt;/b&gt;&lt;b&gt;n&lt;/b&gt;&lt;b&gt;cl&lt;/b&gt;&lt;b&gt;usion:&lt;/b&gt; These results revealed that resistance of &lt;i&gt;Klebsiella&lt;/i&gt; spp. to cephalosporins is high and this may be exacerbated by co-expression of AmpC and
%K &lt
%K i&gt
%K Klebsiella&lt
%K /i&gt
%K Spp.
%K Multidrug Resistance
%K ESBL
%K AmpC
%K Carbapenemase
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=132726