%0 Journal Article
%T Identification and Validation of Novel Biomarkers Related to the Calcium Metabolism Pathway in Hypertension Patients Based on Comprehensive Bioinformatics Methods
%A Xiangguang Chang
%A Lei Guo
%A Liying Zou
%A Yazhao Ma
%A Jilin Feng
%J Health
%P 173-186
%@ 1949-5005
%D 2024
%I Scientific Research Publishing
%R 10.4236/health.2024.163015
%X <b>Background:</b> Hypertension is a universal
risk factor for cardiovascular diseases and is thus the leading cause of death
worldwide. The identification of <span>novel
prognostic and pathogenesis biomarkers plays a key role in diseas</span>e <span>management. <b>Methods:</b> The GSE145854 and
GSE164494 datasets were</span> downloa<span>ded
from the Gene Expression Omnibus (GEO) database and used f</span>or s<span>creening and validating hypertension signature
genes, respectively. Gen</span>e Ontology (GO) enrichment analysis was
performed on the differentially expressed genes (DEGs) related to calcium ion
metabolism in patients with hypertension. The core genes related to immune
infiltration were analyzed and screened, and the activity of the signature
genes and related pathways was quantified using gene set enrichment analysis
(GSEA). The infiltration of immune cells in the blood samples was analyzed, and
the DEGs that were abnormally expressed in the clinical blood samples of
patients with hypertension were verified via RT<span style=\"font-size:10.0pt;font-family:;\" \"=\"\">-</span><span style=\"font-size:10.0pt;font-family:;\" \"=\"\">qPCR. <b>Results:</b> A total of 176 DEGs were screened. <span>GO showed
that DEGs was involved in the regulation of calcium ion met</span>abol<span>ism in biological processes (BP), actin mediated
cell contraction, negative</span> regulation of cell movement, and calcium ion
transmembrane transport, and i<span>n </span>the
regulation of protease activity in molecular functions (MF). KEGG analy<span>sis revealed that the DEGs were involved mainly in
the cGMP</span></span><span style=\"font-size:10.0pt;font-family:;\" \"=\"\">-</span><span style=\"font-size:10.0pt;font-family:;\" \"=\"\">PKG signalin</span><span style=\"font-size:10.0pt;font-family:;\" \"=\"\">g pathway, ubiquitin-protein transferase, tight junction-associated pro</span><span style=\"font-size:10.0pt;font-family:;\" \"=\"\">teins, </span><span style=\"font-family:\" font-size:10pt;\"=\"\">and the
regulation of myocardial cells. MF analysis revealed the</span><span style=\"font-family:\" font-size:10pt;\"=\"\"><span style=\"font-family:;\" \"=\"\"> </span></span><span style=\"font-family:\" font-size:10pt;\"=\"\"><span style=\"font-family:;\" \"=\"\">immune infiltration function of the cells. RT</span></span><span style=\"font-family:\" font-size:10pt;\"=\"\"><span style=\"font-family:;\" \"=\"\">-</span></span><span style=\"font-family:\" font-size:10pt;\"=\"\"><span style=\"font-family:;\" \"=\"\">qPCR revealed that the expression of
%K Hypertension
%K Biomarkers
%K Differentially Expressed Genes
%K Ca&lt
%K sup&gt
%K 2+&lt
%K /sup&gt
%K Metabo-lism
%K Bioinformatics Analysis
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=131783