%0 Journal Article
%T 3,6-Bis-<i>¦Â</i>-Dicarbonylsubstituted Carbazoles Bearing N-Spacers and Their Eu(III) Complexes as Immunofluorescent Labelling Agents
%A Dmitry E. Pugachev
%A Georgy V. Zatonsky
%A Tatyana S. Kostryukova
%A Anna G. Shubina
%A Nikolay V. Vasiliev
%J International Journal of Organic Chemistry
%P 20-31
%@ 2161-4695
%D 2024
%I Scientific Research Publishing
%R 10.4236/ijoc.2024.141002
%X New reagents for immunofluorescence analysis of carbazole series containing fluorinated <i>¦Â</i>-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu<sup>3+</sup> ions possess stability in the aqueous phase, intense and prolonged luminescence (<i>¦Ó</i> 550 - 570 ¦Ìs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region.
%K Fluorescence Immunoassay
%K Fluorinated &lt
%K i&gt
%K ¦Â&lt
%K /i&gt
%K -Diketones
%K Carbazole
%K Europium Complexes
%K Streptavidin
%K Nanodispersions
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=131771