%0 Journal Article
%T Effect of Ultraviolet Radiation on Hsp70 Protein Expression in HaCaT Cells
%A Sergio Hugo S¨¢nchez Rodr¨ªguez
%A Jes¨²s Rodr¨ªguez Vergil
%A Manuel Venancio Mu£¿oz Ju¨¢rez
%A Kevin Said Ram¨ªrez D¨¢vila
%A Luis Mart¨ªn Garc¨ªa Ortiz
%A Germ¨¢n Flores Cort¨¦s
%A Luz Elena Vidales Rodr¨ªguez
%A Jes¨²s Adri¨¢n L¨®pez
%A David Alejandro Garc¨ªa L¨®pez
%J World Journal of Nuclear Science and Technology
%P 59-71
%@ 2161-6809
%D 2024
%I Scientific Research Publishing
%R 10.4236/wjnst.2024.141002
%X Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with humans to a greater or lesser extent, and can generate adverse effects such as cellular stress when interacting with intra-and extracellular biomolecules. The skin is the first organ in contact with UV radiation, and the stress it generates can be analyzed by the expression of a bioindicator of cellular damage such as Hsp70. Therefore, the objective of the project was: to determine the effect of UVA, UVB and UVC radiation on HaCaT epithelial cells, by analyzing the expression of Hsp70. Materials and methods: HaCaT cells were cultured <em>in vitro</em>, which were irradiated with UVA, UVB and UVC light at different doses, to subsequently determine the degree of Hsp70 expression by Immunodetection by PAGE-SDS and Western Blot. Results: Basal expression of Hsp70 was observed in no irradiated HaCaT cells. When HaCaT cells were irradiated with UVA, UVB, UVC, an increase in this Hsp70 protein was observed. With UVA, a higher degree of expression was observed at a time of 30 minutes of irradiation. With UVB the highest expression shifted to a time of 20 minutes. With UVC, overexpression was observed after 10 minutes. Conclusion: UV radiation generates cellular stress on HaCaT cells, evaluated by the stress bioindicator Hsp70. According to the wavelength of UV radiation, those that have a shorter wavelength have a greater potential for cellular damage, such as UVC.
%K Ultraviolet A Light (UVA)
%K Ultraviolet B Light (UVB)
%K Ultraviolet C Light (UVC)
%K Heat Shock Protein 70 (Hsp70)
%K HaCaT
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=130628