%0 Journal Article
%T Extracellular Vesicles from Mesenchymal Stromal Cells (imEVs) Improve Cold Preservation of Isolated Mitochondria
%A Xianpeng Jiang
%A Sergey Rodin
%A Ken Braesch-Andersen
%A Catherine C. Baucom
%A Karl-Henrik Grinnemo
%A Brent Segal
%J Journal of Biosciences and Medicines
%P 52-63
%@ 2327-509X
%D 2024
%I Scientific Research Publishing
%R 10.4236/jbm.2024.121005
%X Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, Parkinson¡¯s diseases, brain and spinal cord injuries, and amyotrophic lateral sclerosis (ALS). However, one of the major challenges for widespread usage is a methodology for preservation of isolated mitochondria. Extracellular vesicles (EVs) are phospholipid bilayer-enclosed vesicles released from cells. EVs carry a cargo of proteins, nucleic acids, lipids, metabolites, and even organelles such as mitochondria. <strong>Purpose: </strong>To test if EVs enhance the stability of isolated mitochondria. <strong>Methods:</strong> We mixed isolated mitochondria of fibroblasts with EVs of mesenchymal stromal cells (imEVs) (9:1 in volume) and stored the mixture at 2&amp;deg;C - 6<span style=\"text-wrap:wrap;\"><span style=\"text-wrap:wrap;\">&amp;deg;</span></span>C for different time periods. We measured morphology, mitochondrial membrane potential (MMP) and mitochondrial ATP content at 0, 2, 5 days. <strong>Key findings:</strong> After 2 days of storage, the mito-chondria without imEVs lost approximate 70% MMP (RFU: 1822 ¡À 68), compared to the fresh mitochondria (RFU: 5458 ¡À 52) (p &lt; 0.01). However, MMP of the mitochondria mixed with imEVs (RFU: 6786 ¡À 291) was even slightly higher than MMP of the fresh mitochondria (RFU: 5962 ¡À 222) (p &gt; 0.05). In agreement with MMP, mitochondria without imEVs lost significant mitochondrial ATP content (p &lt; 0.01), but the mitochondria with imEVs addi-tion preserved at least 90% mitochondrial ATP (p &gt; 0.05), after 2 days of cold storage, compared to fresh mitochondria. Microscopy showed that imEVs promoted aggregation of isolated mitochondria. <strong>Summary:</strong> The preliminary data showed that imEVs enhanced the stability of isolated mitochondria in cold storage.
%K Mitochondria
%K Extracellular Vesicles
%K Mitochondrial Preservation
%K MOT
%K imEVs
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=130414