%0 Journal Article %T Release of Arabinose from Wheat Insoluble Arabinoxylan by the Action of <i>¦Á</i>-L-Arabinofuranosidase in Synergism with Endo-Xylanases %A Dominic W. S. Wong %A Sarah Batt %J Advances in Enzyme Research %P 147-153 %@ 2328-4854 %D 2023 %I Scientific Research Publishing %R 10.4236/aer.2023.114007 %X Wheat arabinoxylan (water-insoluble fraction) contains ~36% arabinose which may include both singly or doubly substitutions at C2/C3 of the Xylp units. ¦Á-L-Arabinofuranosidses (ABFs) of two GH families were analyzed for their respective activities on the hydrolysis of Xylp-Araf. BaABF (GH43) produced twice the yield of arabinose residues from the heteroxylan compared to AnABF (GH51) under the same reaction conditions. Two endo-xylanases (of GH10 and 11) also showed differential hydrolytic activities on the Xylp chain, with the GH10 XYN-ATM double the amount of reducing sugar yield (as xylose equivalent) than using the GH11 XYN-M3. When the ABF and XYN were combined in optimial ratios, a synergistic increase of 73.8% in arabinose yield was observed. %K < %K i> %K ¦Á< %K /i> %K -L-Arabinofuranosidase %K Mono-Substituted Xylopyranosyl Residuet %K Di-Substituted Xylopyranosyl Unit %K Arabinoxylan Arabinofuranohydrolase (AXH) %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=130217