%0 Journal Article
%T Release of Arabinose from Wheat Insoluble Arabinoxylan by the Action of <i>¦Á</i>-L-Arabinofuranosidase in Synergism with Endo-Xylanases
%A Dominic W. S. Wong
%A Sarah Batt
%J Advances in Enzyme Research
%P 147-153
%@ 2328-4854
%D 2023
%I Scientific Research Publishing
%R 10.4236/aer.2023.114007
%X Wheat arabinoxylan (water-insoluble fraction) contains ~36% arabinose which may include both singly or doubly substitutions at C2/C3 of the Xyl<em>p</em> units. <em>¦Á</em>-L-Arabinofuranosidses (ABFs) of two GH families were analyzed for their respective activities on the hydrolysis of Xyl<em>p</em>-Araf. BaABF (GH43) produced twice the yield of arabinose residues from the heteroxylan compared to AnABF (GH51) under the same reaction conditions. Two endo-xylanases (of GH10 and 11) also showed differential hydrolytic activities on the Xyl<em>p</em> chain, with the GH10 XYN-ATM double the amount of reducing sugar yield (as xylose equivalent) than using the GH11 XYN-M3. When the ABF and XYN were combined in optimial ratios, a synergistic increase of 73.8% in arabinose yield was observed.
%K &lt
%K i&gt
%K ¦Á&lt
%K /i&gt
%K -L-Arabinofuranosidase
%K Mono-Substituted Xylopyranosyl Residuet
%K Di-Substituted Xylopyranosyl Unit
%K Arabinoxylan Arabinofuranohydrolase (AXH)
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=130217