%0 Journal Article %T 龟甲胶的快速制备工艺及其抗氧化活性研究
The Rapid Production Method and Antioxidant Activity of Turtle-Shell Glue %A 刘沁烨 %A 蔡嘉玲 %A 谈笑 %A 曹晖 %J Hans Journal of Food and Nutrition Science %P 276-284 %@ 2166-6121 %D 2023 %I Hans Publishing %R 10.12677/HJFNS.2023.124034 %X 以中华草龟为原料,探讨超高压技术对龟甲胶出胶率、提取时间、抗氧化活性的影响。采用300 MPa-15 min、300 MPa-30 min、500 MPa-15 min、500 MPa-30 min四种超高压条件对龟甲进行预处理制备龟甲胶,并测定其抗氧化活性。结果显示,与传统制备工艺相比,超高压技术可大大提高龟甲胶的生产效率,其中300 MPa-30 min超高压处理组的提取时间可缩短42%。此外,不同方法制备的龟甲胶皆具有抗氧化活性,四种超高压处理组对?OH的清除率均高于传统工艺组,最高达37.39%;500 MPa-15 min、300 MPa-15 min、500 MPa-30 min组对DPPH?的清除率高于传统工艺组,最高达76.76%;500 MPa-15 min、500 MPa-30 min组对ABTS+?的清除率高于传统工艺组,最高达97.33%。本研究可为龟甲胶的快速制备工艺提供理论依据。
In this study, Chinese turtles were served as the experimental material to analyze the effect of ultra-high pressure on the yield, extraction time and antioxidant activity of turtle-shell glue. The turtle-shell were pretreated by four kinds of ultra-high pressure treatments: 300 MPa-15 min, 300 MPa-30 min, 500 MPa-15 min and 500 MPa-30 min, which were made into turtle-shell glue afterwards. The scavenging activity between turtle-shell glue made by ultra-high pressure and the traditional method was detected as well. The results showed that, in comparison with the traditional method, ultra-high pressure could greatly improve the production efficiency, and the extraction time of 300 MPa-30 min group could be reduced by 42%. Additionally, the turtle-shell glue made by different methods all had antioxidant activity. The clearance rates of ?OH of four ultra-high pressure groups were higher than that of the traditional group, with a maximum of 37.39%; the clearance rates of DPPH? of 500 MPa-15 min, 300 MPa-15 min, and 500 MPa-30 min groups were higher than that of the traditional group, with a maximum of 76.76%; the clearance rates of ABTS+? of 500 MPa-15 min and 500 MPa-30 min groups were higher than that of the traditional group, with a maximum of 97.33%. This study can provide a theoretical basis for the rapid production method of turtle-shell glue. %K 龟甲胶,超高压,胶原蛋白,抗氧化活性
Turtle-Shell Glue %K Ultra-High Pressure %K Collagen %K Antioxidant Activity %U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=74442