%0 Journal Article
%T Detection of Human Papillomavirus DNA and E6/E7 mRNA from HPV Genotypes 16, 18, 31 and 33 in Histologically Confirmed Cases of Cervical Cancer and Precancerous Lesions in Burkina Faso
%A Th¨¦odora Mahouk¨¨d¨¨ Zohoncon
%A Shoukrat Ohuwa Toyin Bello
%A Prosper Bado
%A Rogomenoma Alice Ou¨¦draogo
%A Estelle Ou¨¦draogo
%A Ina Marie Ang¨¨le Traor¨¦
%A Abdoul Karim Ouattara
%A Florencia Wenkunni Djigma
%A Albert Th¨¦ophane Yonli
%A Assita Sanou-Lamien
%A Olga M¨¦lanie Lompo
%A Jacques Simpore
%J American Journal of Molecular Biology
%P 276-290
%@ 2161-6663
%D 2023
%I Scientific Research Publishing
%R 10.4236/ajmb.2023.134019
%X <b>Introduction:</b> Cervical cancer, caused by persistent high-risk human papillomavirus (HPV)
infection, remains a global public health problem. The cellular transformation
and maintenance of the malignant phenotype of these HPVs are attributed to the
viral oncoproteins E6 and E7. <b>Objective:</b> This study aims to detect the
presence of human papillomavirus DNA and E6/E7 oncoprotein mRNA of HPV
genotypes 16, 18, 31 and 33 in cases of cervical cancer and precancerous
lesions, histologically confirmed in Burkina Faso. <b>Methods:</b> This
descriptive cross-sectional study focused on cases of cervical cancer and
high-grade intraepithelial neoplasia (CIN) and was conducted from June to
December 2022. One hundred (100) samples of fixed and paraffin-embedded tissues
were collected from the pathological anatomy and cytology laboratories of
hospitals in the capital of Burkina Faso. High-risk human papillomavirus
(HR-HPV) DNA was detected using multiplex real-time PCR, while the presence of
E6 and E7 mRNA in cervical cancer and high-grade CIN samples was determined
using real-time Reverse Transcriptase-PCR (RT-PCR) with TaqMan probes. <b>Results:</b> The mean age of women diagnosed with cervical cancer and high-grade CIN was
50.81 ¡À 13.65 years, ranging from 22 to 82 years. Cervical cancer and
high-grade CIN were positive for at least one high-risk human papillomavirus
(HR-HPV) in 80% of cases. The most prevalent genotypes observed were HPV16, 18,
31, and 33, collectively accounting for 70.08% of cases. Of the 89 samples that
tested positive for HR-HPV genotypes 16, 18, 31, and 33, 88 (98.88%; 95% CI: [94.58 - 99.94]) were also
positive for the presence of mRNA encoding the E6 and E7 oncoproteins of HPV16,
18, 31, and 33. <b>Conclusion:</b> In the presence of HPV DNA, testing for E6
and E7 oncoprotein mRNA could serve as a promising biomarker and valuable tool
for improved assessment of the progression to cervical cancer.
%K HPV
%K E6/E7
%K Cervical Cancer
%K Precancerous Lesions
%K Burkina Faso
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=128469