%0 Journal Article
%T CXCR5<sup>+</sup> CD8<sup>+</sup> T细胞在儿童传染性单核细胞增多症中的反应特点及临床价值<br>Responding Characteristics and Clinical Value of CXCR5<sup>+</sup> CD8<sup>+</sup> T Cells in Children with Infectious Mononucleosis
%A 薛健
%A 王建勇
%A 张晓录
%A 谷钰峰
%A 邢海燕
%A 辛毅
%A 王晓莉
%A 李爱敏
%J Advances in Clinical Medicine
%P 8145-8155
%@ 2161-8720
%D 2023
%I Hans Publishing
%R 10.12677/ACM.2023.1351140
%X 目的：本研究旨在探讨C-X-C趋化因子受体5型(CXCR5) CD8<sup>+</sup> T细胞在儿童EBV相关传染性单核细胞增多症(IM)中的反应特点及临床价值。方法：采用流式细胞术及EB病毒(EBV)病原学检查分析入组儿童的外周血，同时收集临床资料进行统计分析。结果：EBV感染急性期CXCR5<sup>+</sup> CD8<sup>+</sup> T细胞绝对值计数显著增加，CXCR5<sup>+</sup> CD8<sup>+</sup> T/CD8<sup>+</sup> T细胞比值、CD19<sup>+</sup> B细胞的绝对值计数及CD19<sup>+</sup> B细胞/LYM比值显著降低。CXCR5<sup>+</sup> CD8<sup>+</sup> T细胞绝对值计数与EBV DNA病毒载量、CD19<sup>+</sup> B细胞绝对值计数呈负相关与肝损伤指标呈正相关。将EBV相关性IM急性期患儿以CXCR5<sup>+</sup> CD8<sup>+</sup> T细胞绝对值计数中位值分为高、低表达两组，高表达组中出现淋巴结肿大、肝脾肿大的几率明显高于低表达组。ROC曲线分析显示CXCR5<sup>+</sup> CD8<sup>+</sup> T细胞对儿童IM具有诊断价值，对于EBV相关肝损伤具有预测价值。结论：CXCR5<sup>+</sup> CD8<sup>+</sup> T细胞在EBV的急性感染中发挥了抗病毒作用，而其过度反应可能导致组织器官损伤。此外，CXCR5<sup>+</sup> CD8<sup>+</sup> T细胞可作为EBV相关IM诊断的诊断指标及EBV相关性肝损伤的预测指标。<br />
Objective: To investigate the response characteristics and clinical value of C-X-C chemokine receptor type 5 (CXCR5) CD8<sup>+</sup> T cells in children with EBV-associated infectious mononucleosis (IM). Methods: Peripheral blood of the enrolled children was analyzed by flow cytometry and Epstein-Barr virus (EBV) etiology, and clinical data were collected for statistical analysis. Results: The absolute number of CXCR5<sup>+</sup> CD8<sup>+</sup> T cells increased significantly, the CXCR5<sup>+</sup> CD8<sup>+</sup> T/CD8<sup>+</sup> T cell ratio, the absolute number of CD19<sup>+</sup> B cells and the CD19<sup>+</sup> B cell/LYM ratio decreased significantly in the acute stage of EBV infection. The absolute number of CXCR5<sup>+</sup> CD8<sup>+</sup> T cells was negatively correlated with EBV DNA load and absolute number of CD19<sup>+</sup> B cells, and positively correlated with liver injury index. The children in the acute stage of EBV-associated IM were divided into high and low expression groups according to the median value of CXCR5<sup>+</sup> CD8<sup>+</sup> T cells absolute number. The probability of lym-phadenectasis, hepatosplenomegaly in the high expression group was significantly higher than that in the low expression group. ROC curve analysis showed that CXCR5<sup>+</sup> CD8<sup>+</sup> T cells had diagnostic value in EBV-associated IM, and had predictive value in EBV-related liver injury. Conclusion: CXCR5<sup>+</sup> CD8<sup>+</sup> T cells act as an antiviral role in acute infection of EBV, and their overreaction may lead to tis-sue and organ damage. In addition, CXCR5<sup>+</sup> CD8<sup>+</sup> T cells can be used as diagnostic indicators of EBV-associated IM and predictive indicators of EBV-associated liver injury.
%K CXCR5<sup>+</sup> CD8<sup>+</sup> T细胞，EBV相关性IM，流式细胞术，EBV相关性肝损伤<br>CXCR5<sup>+</sup> CD8<sup>+</sup> T Cells
%K EBV-Associated IM
%K Flow Cytometry
%K EBV-Associated Liver Injury
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=65657