%0 Journal Article
%T Prevalence and Spatial Distribution of Badnavirus in the Banana (<i>Musa</i> spp) Major Growing Areas in Burkina Faso
%A Bakary Ouattara
%A Drissa S¨¦r¨¦m¨¦
%A Moustapha Koala
%A L¨¦on W¨ºnd¨¦-m¡¯min¨¨r¨¦ Niti¨¦ma
%A Kadidia Ko£¿ta
%A Emmanuel Kabor¨¦
%A Issa Wonni
%J American Journal of Plant Sciences
%P 427-447
%@ 2158-2750
%D 2023
%I Scientific Research Publishing
%R 10.4236/ajps.2023.144028
%X Banana streak virus (BSV) and Sugarcane bacilliform virus (SCBV) are two
badnaviruses commonly found in all banana growing areas of the world. It is a
threat to the production and improvement of <i>Musa</i> germplasm. In Burkina
Faso, the presence of badnaviruses was reported in banana producing regions.
The objective of this study was to determine the prevalence of BSV and SCBV in
banana production areas of Burkina Faso. A survey followed by a symptomatologic
study was conducted in banana plantations in 27 localities of the nine main
banana producing regions from July to October 2018 and September to December 2020.
In all, 251 leaf samples were collected and analysed for BSV and SCBV infection
by Indirect Antigen Coated Plate Assay-ELISA followed by amplification of the
RT/RNase H region using Polymerase chain reaction with Badna FP/RP and SCBV F/R
primers, respectively. A variety of symptoms were observed on almost all plant
organs which were revealed due to BSV by symptomatologic study. The results of
serological and molecular diagnosis revealed a high overall prevalence of BSV
in 80.48% of the samples tested. BSV was distributed in seven survey regions
out of nine with prevalence ranging from 10% to 100% in North, Centre, Centre
West, Hauts Bassins, Cascades, Centre East and Boucle of Mouhoun regions. Very
low prevalence was recorded for SCBV in Cascades and East Centre region with
4.35 and 12.5%, respectively. Species detection using specific primers to each
species revealed three main species: Banana streak Obino l¡¯ewa<span style=\"white-space:nowrap;\">&#239;</span> virus (BSOLV),
Goldfinger virus (BSGFV) and Imov¨¦ virus (BSIMV) in the samples tested, respectively
in the proportions of 23%, 8% and 0.8%. Co-infection between BSV species was
also detected.
%K Banana Streak Virus
%K Sugarcane Bacilliform Virus
%K Indirect Antigen Coated Plate Assay
%K Polymerase Chain Reaction
%K &lt
%K i&gt
%K Musa&lt
%K /i&gt
%K spp
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=124175