%0 Journal Article
%T Determination of Alpha-Lipoic Acid in a Nutritional Supplement Using High Performance Liquid Chromatography
%A Trapali Maria
%A Fotia Eirini
%J Journal of Diabetes Mellitus
%P 216-223
%@ 2160-5858
%D 2022
%I Scientific Research Publishing
%R 10.4236/jdm.2022.124017
%X Alpha lipoic acid has the ability to react and neutralize reactive oxygen species (ROS) such as superoxide radicals, simple oxygen, hydroxyl radicals, hypochlorous acid and peroxyl radicals. A rapid high-performance liquid chromatographic method for determination of lipoic acid in a nutritional supplement was developed. The method involved sample preparation and the mobile phase comprised of 50 mM disodium hydrogen phosphate buffer (pH 2.5 adjusted with 1 M H<sub>3</sub>PO<sub>4</sub>): acetonitrile in the ratio of 50:50. The separation was done using a C18 column (150 mm) and detection was carried out using UV detection at 201 nm. The assay was found to be linear in the range of 1.56 - 50 ¦Ìg/mL with the correlation coefficient of 0.9997. Method precision was determined while LOD was 0.05 ¦Ìg/mL and LOQ 0.15 ¦Ìg/mL. The chromatographic peak LA retention time was 6 min.
%K Alpha-Lipoic Acid
%K HPLC
%K Antioxidant Activity
%K Nutritional Supplement
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=121008