%0 Journal Article
%T Molecular Characterization and Phylogenetic Relationships of Melon (Cucumis melo L. Meloni Cultivar) Based on Expression of Andromonoecious Gene
%A Eka Wasi¡¯ Al-Mughni
%A Budi Setiadi Daryono
%J HAYATI Journal of Biosciences
%P 174-179
%@ 2086-4094
%D 2020
%R 10.4308/hjb.27.2.174
%X Melon (Cucumis melo L.) is one of horticultural commodities with good economic prospect for melon farmers because of several advantages. 'Meloni' is result of crossing between ¡®Sun Lady-3¡¯ and ¡®PI 371795¡¯ by Genetics and Breeding Laboratory, Faculty of Biology, UGM. The 'Meloni' plant produces females flowers that expressed by CmACS-7 gene and it can be expected that melon plants 'Meloni' included in the primitive cultivars, while 'Meloni' is a cultivation cultivar. The aim of this research is to study the relation of 'Meloni' kinship to primitive and modern cultivars. Characterization of the CmACS-7 gene is begun by a total isolation process of RNA from a sample of melon plant flowers and detection using a specific primer. The cDNA bands obtained are sequenced and then phylogenetic analysis is performed based on CmACS-7 gene expression using MEGA 7.0. Result of this study show that the presence of the CmACS-7 gene in female and hermaphrodite flowers of ¡®Meloni¡¯, ¡®PI 371795¡¯, ¡®Sun Lady-3¡¯, and ¡®Hikapel¡¯. Phylogenetic tree analyze show that ¡®Meloni¡¯ from female plant, ¡®PI 371795¡¯ and ¡®Sun Lady-3¡¯ have closely relation, while 'Hikapel' separated from other cultivars.
%K Cucumis melo L.
%K Meloni
%K CmACS-7 gen
%K aminocyclopropane carboxylic acid synthase
%K phylogenetic
%U https://journal.ipb.ac.id/index.php/hayati/article/view/31501