%0 Journal Article
%T Buds Reactivity and Factors Promoting Shoots Proliferation and Rooting of Cashew Seedlings Using <i>in Vitro</i> Tissue Culture Process
%A B¨¦n¨¦dicte Sophie Adjoua Akakpo
%A Arnaud Agbidinoukoun
%A Corneille Ahanhanzo
%A Gilles Habib Todjro Cacai
%A Bienvenu T¨¦midouan Badou
%A Serge Houedjissin
%A Joseph Dossou
%J Advances in Bioscience and Biotechnology
%P 388-400
%@ 2156-8502
%D 2022
%I Scientific Research Publishing
%R 10.4236/abb.2022.139025
%X Tissue culture techniques are widely used for the
mass propagation of many species. In cashew <i>in
vitro</i> propagation, some protocols need to be established at this end. The
present work was carried out to evaluate the conditions for <i>in vitro </i>regeneration of cashew
seedlings from micropropagation by organoge<span>nesis
on Benin genotypes. Nodal explants from one-month-old cashew se</span>edlings
in the greenhouse and cotyledonary nodes from <i>in vitro </i>germination were used for this purpose. BAP and kinetin
were evaluated alone at 2.2 mg/L and then the combination of 2.2 mg/L BAP + 0.2
mg/L IBA was also evaluated. The response of axillary bud proliferation on
explants was obtained with both cotyledonary nodes and axillary buds from
different combinations of growth regulators. However, the best responses were
recorded with cotyledonary nodes. When 2.2 mg/L BAP was used, 80% of the
explants responded with numerous proliferation (5 to 8) buds (5.75 ¡À 0.12) with
good shoot length (6.73 ¡À 0.3 cm) on MS medium containing 150 mL coconut water.
Rooting was observed with the combination of NAA (2.5 mg/l) + IBA (2.5 mg/l) on
1/2 MS containing 40 g/l sucrose.
%K Micropropagation
%K Cashew Elite Genotypes
%K Buds
%K &lt
%K i&gt
%K In Vitro&lt
%K /i&gt
%K Regeneration
%K Organogenesis
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=119912