%0 Journal Article
%T HPLC测定不同产地南板蓝根中靛玉红含量<br>Determination of Indirubin in Baphicacanthis Cusiae Rhizoma et Radix from Different Habitats by HPLC
%A 周芳丽
%A 张丽艳
%A 李军
%J Hans Journal of Medicinal Chemistry
%P 198-203
%@ 2331-8295
%D 2022
%I Hans Publishing
%R 10.12677/HJMCe.2022.102019
%X 目的：建立不同产地南板蓝根药材中靛玉红含量的高效液相色谱法。方法：采用Agilent ZORBAX SB-C<sub>18</sub>色谱柱(5 μm, 4.6 × 150 mm)；以甲醇-0.1%磷酸水为流动相，梯度洗脱；流速为0.8 mL·min<sup>？1</sup>；柱温为35℃；检测波长为290 nm；进样量为10 μL。结果：靛玉红的进样量在0.63~12.6 μg (r = 0.9999)范围内与峰面积呈较好的线性关系；平均回收率为101.18% (RSD为2.74%)；靛玉红的含量为0.040~0.151 mg·g<sup>？1</sup>。结论：建立的南板蓝根含量测定方法操作简单、具有良好的重复性和可靠性。对南板蓝根质量控制及整体性评价具有一定的指导意义。&lt;br&gt;Objective: To establish an HPLC method for the content of indirubin in Baphicacanthis Cusiae Rhi-zoma et Radix from different habitats. Methods: Agilent ZORBAX SB-C18 column (5 μm, 4.6 × 150 mm) was used. Gradient elution was performed with methanol-0.1% phosphoric acid as mobile phase. The flow rate was 0.8 mL·min&lt;sup&gt;？1&lt;/sup&gt;. The column temperature was 35？C. The detection wave-length was 290 nm. The injection volume was 10 μL. Results: The sample amount of indirubin showed a good linear relationship with the peak area in the range of 0.63~12.6 μg (r = 0.9999). The average recovery rate was 101.18% (RSD 2.74%). The content of indirubin was determined in the range of 0.040~0.151 mg·g&lt;sup&gt;？1&lt;/sup&gt;. Conclusion: The established method for the determination of Baphic-acanthis Cusiae Rhizoma et Radix is simple, reproducible and reliable. It has certain guiding signif-icance for quality control and integrity evaluation of Baphicacanthis Cusiae Rhizoma et Radix.<br />
%K 南板蓝根，高效液相色谱，靛玉红，含量测定<br>Baphicacanthis Cusiae Rhizoma et Radix
%K High Performance Liquid Chromatography
%K Indirubin
%K Content Determination
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=51934