%0 Journal Article
%T Caveolin-1 Inhibits Proliferation and Migration of Gastric Cancer Cell via Inactivating BMI-1
%A Jun Wang
%A Shasha Liu
%J Open Journal of Pathology
%P 80-87
%@ 2164-6783
%D 2022
%I Scientific Research Publishing
%R 10.4236/ojpathology.2022.123010
%X <strong>Objective: </strong>To investigate the effect of Caveolin-1on the proliferation and migration ability in gastric carcinoma cells MGC-803 and its mechanism. <strong>Methods:</strong> Plasmid DNA pcDNA3.1-Cav1 was extracted by extracted kits, and transfected Cav-1 gene sequences were found. The expression levels of Cav-1 protein were detected by Western blot. And the proliferation was analyzed by CCK8 assay. The effect of Cav-1 on migration was detected by wound healing. The expression levels of BMI-1 protein were detected by western blot. <strong>Results:</strong> 1) Western Blot showed that the expression levels of Cav-1 were higher in MGC-803/Cav-1 than control group, P &lt; 0.05. 2) CCK8 showed, that the proliferation of MGC-803/Cav-1 was inhibited, but P &gt; 0.05. Wound healing showed, that the migration ability of MGC-803/Cav-1 fell off, P &lt; 0.05. 3) Western Blot showed that the expression levels of BMI-1 were lower in MGC-803/Cav-1 than in the control group, P &lt; 0.05. <strong>Conclusion: </strong>Caveolin-1 can inhibit the proliferation and migration ability of gastric carcinoma cells and its mechanism may relate to BMI-1.
%K Caveolin-1
%K Proliferation
%K Migration
%K BMI-1
%K GC
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=117082