%0 Journal Article
%T 基于2,6-二-(2-吡啶基)-4-吡啶甲酸的紫外法检测辣根过氧化物酶浓度<br>Determination of Horseradish Peroxidase Concentration Based on 2,6-Di-(2-pyridyl)-4-pyridyl Carboxylic Acid by UV Method
%A 蔡燕
%A 严鑫
%A 王泽东
%A 姚勇
%J Advances in Analytical Chemistry
%P 261-267
%@ 2163-1565
%D 2021
%I Hans Publishing
%R 10.12677/AAC.2021.114028
%X 本文首次提出采用2,6-二-(2-吡啶基)-4-吡啶甲酸(配体L)-H<sub>2</sub>O<sub>2</sub>-HRP测定HRP酶浓度的新体系。利用紫外考查HRP催化H<sub>2</sub>O<sub>2</sub>氧化配体L体系的光谱变化。在pH = 7.2，25℃下，配体L浓度为6 × 10<sup>？6</sup> mol/L，H2O2浓度为1 × 10<sup>？4 </sup>mol/L时，回归方程为A = ？0.0034x + 1.072，线性相关系数为0.9909，该体系的检测范围可达10<sup>？8</sup>~10<sup>？9</sup> mol/L，灵敏度较高，操作简便，抗干扰能力强，稳定性好，可用作检测游离HRP酶的新方法，具有实际的应用前景。<br />
A new system for the determination of horseradish peroxidase concentration by 2,6-di-(2-py- ridyl)<sup>-4</sup>-picolinic acid (ligand L)-H<sub>2</sub>O<sub>2</sub>-HRP was proposed for the first time. The spectral changes of ligand L system catalyzed by HRP for H<sub>2</sub>O<sub>2</sub> oxidation were investigated by UV method. At pH = 7.2 and 25？C, the ligand L concentration is 6 × 10<sup>？6</sup> mol/L, H<sub>2</sub>O<sub>2</sub> concentration is 1 × 10<sup>？4</sup> mol/L, the regression equation is A = ？0.0034x + 1.072 and the linear correlation coefficient is 0.9909. The detection range of the system can reach 10<sup>？8</sup>~10<sup>？9</sup> mol/L. This method has high sensitivity, simple operation, strong anti-interference ability and good stability. It can be used as a new method for the detection of free HRP enzyme and has practical application prospects.
%K 辣根过氧化物酶，2
%K 6-二-(2-吡啶基)-4-吡啶甲酸，紫外，浓度，水相<br>Horseradish Peroxidase
%K 2
%K 6-Di-(2-pyridyl)-4-picolinic Acid
%K Ultraviolet
%K Concentration
%K Aqueous Phase
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=46423