%0 Journal Article
%T 基于整合药理学的乳酸改善小鼠焦虑作用及机制研究<br>Research on the Effect and Mechanism of Lactic Acid on Improving Anxiety in Mice Based on Integrated Pharmacology
%A 赵金龙
%A 邓子璇
%A 蔡焕宇
%A 陈莆苑
%A 贺志明
%J International Journal of Psychiatry and Neurology
%P 99-107
%@ 2166-580X
%D 2021
%I Hans Publishing
%R 10.12677/IJPN.2021.104013
%X 目的：基于网络药理学方法探究乳酸改善焦虑的主要靶点及信号通路,结合行为学初步研究其潜在作用机制方法：通过Swiss Target Prediction、Target Net、Sea数据库获得乳酸作用靶点；通过Gene Cards、OMIM、CTD数据库获得焦虑靶点；通过Gene Cards和OMIM建立乳酸和焦虑症的靶基因集用韦恩图显示；通过STRING数据库构建乳酸与焦虑蛋白互作PPI网络图，利用DAVID数据库进行GO功能富集与KEGG通路富集分析。基于网络药理学预测的通路结果，将雄性ICR小鼠随机成溶媒组、乳酸组，每组10只，进行旷场实验，在实验前1 h腹腔注射乳酸或溶媒，记录在旷场实验中进入中间区域的时间和活动度并计算辨别指数。结果：通过Swiss Target Prediction、Target Net、Sea数据库共获得乳酸作用靶点248个；通过Gene Cards、OMIM、CTD数据库共获得焦虑靶点717个；靶点经与Gene Cards和OMIM综合数据库比对，共获取28个乳酸与焦虑的交集基因。结论：乳酸主要通过调控MAO-A信号通路进一步调节5-HT代谢，以及调控苯丙氨酸和酪氨酸代谢与cAMP信号通路及相应的基因表达进一步调节儿茶酚胺类神经递质代谢发挥抗焦虑作用。<br />
Objective: To explore the main targets and signal pathways of lactic acid for improving anxiety based on the method of network pharmacology, and to study its potential mechanism of action in combination with behavioral studies. Methods: Obtain lactic acid targets through Swiss Target Prediction, Target Net, and Sea databases; obtain anxiety targets through Gene Cards, OMIM, and CTD databases; establish lactic acid and anxiety target gene sets through Gene Cards and OMIM; display with Venn diagram; construct lactic acid through STRING database. It interacts with anxiety protein as a PPI network diagram, and uses DAVID database for GO function enrichment and KEGG pathway enrichment analysis. Based on the results of network pharmacology, Male ICR mice were randomly divided into a vehicle group and a lactic acid group, each with 10 mice, and an open field experiment was performed. Lactic acid or vehicle was injected into the abdominal cavity 1 hour before the experiment and the time and activity of entering the middle area in the open field experiment were recorded and the discrimination was calculated. Index. Results: A total of 248 lactic acid targets were obtained through Swiss Target Prediction, Target Net, and Sea databases; a total of 717 anxiety targets were obtained through Gene Cards, OMIM, and CTD databases; a total of 28 lactic acid targets were obtained by comparison with Gene Cards and OMIM comprehensive databases. The intersection gene with anxiety. Conclusion: Lactic acid mainly regulates 5-HT metabolism by regulating the MAO-A signaling pathway, as well as regulating the metabolism of phenylalanine and tyrosine, the cAMP signaling pathway and the corresponding gene expression to further regulate the metabolism of catecholamine neurotransmitters to exert anti-anxiety effects.
%K 乳酸，焦虑，网络药理学，旷场实验<br>Lactic Acid
%K Anxiety Disorder
%K Network Pharmacology
%K Open Field Experiments
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=46201