%0 Journal Article
%T 10 L转瓶培养与10层细胞工厂培养制备麻疹单次病毒收获液的比较<br>Comparison of 10 L Spinner Bottle Cell Culture and 10 Layer Cell Factory Cell Culture in Preparing Measles Virus Single Harvest Fluid
%A 张和平
%A 刘鑫娜
%A 李慧
%A 杨兆荣
%A 李玲洁
%A 王菲
%A 蒿张静
%A 公殿力
%J Hans Journal of Biomedicine
%P 241-246
%@ 2161-8984
%D 2021
%I Hans Publishing
%R 10.12677/HJBM.2021.114031
%X 目的：对10 L转瓶培养与10层细胞工厂培养制备麻疹单次病毒收获液进行比较，评价两种生产工艺的优劣。方法：将10 L转瓶培养与10层细胞工厂培养制备麻疹单次病毒收获液的每一步进行比较，通过关键质量指标的病毒滴度来对其生产工艺进行评价。结果：10 L转瓶培养和10层细胞工厂培养制备麻疹单次病毒收获液的病毒滴度分别为5.6 lgCCID50/mL、5.6 lgCCID50/mL。结论：10 L转瓶培养工艺和10层细胞工厂培养工艺均可以制备麻疹单次病毒收获液，二者没有显著性差异。<br />
Objective: The objective is to compare the advantages and shortages between 10 L spinner bottle cell culture and 10 layer cell factory cell culture in preparing measles virus single harvest fluid process. Methods: In each step, the two processes were assessed by viral titer, a Critical Quality Attribute (CQA) in measles virus harvest production. Results: The viral titer results of 10 L spinner bottle cell culture process and 10 layer cell factory cell culture process are 5.6 lgCCID50/mL and 5.6 lgCCID50/mL respectively. Conclusion: The measles virus single harvest fluid could be prepared by both spinner bottle cell culture and 10 layer cell factory cell culture, but there was no significant difference between them.
%K 麻疹单次病毒收获液，转瓶，细胞工厂，病毒滴度，培养工艺<br>Measles Virus Single Harvest Fluid
%K Spinner Bottle
%K Cell Factory
%K Virus Titer
%K Cultivation Process
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=45987