%0 Journal Article
%T 富含脂肪酶菌种的筛选与鉴定<br>Screening and Identification of Lipase-Rich Strains
%A 李乔惠
%A 王冰鹤
%A 李成龙
%A 杨森
%A 王悦琦
%A 周欢
%A 李海玲
%A 高秀峰
%J Biophysics
%P 65-71
%@ 2330-1694
%D 2021
%I Hans Publishing
%R 10.12677/BIPHY.2021.94008
%X 脂肪酶是生物柴油生产的关键酶，为了降低生物柴油的研发成本，本研究从餐厅油脂污染处和自然环境中筛选得到了含脂肪酶菌；然后对其进行了紫外以及高温诱变。对诱变后的脂肪分解菌利用罗丹明B固体培养基对其活性进行了鉴定；利用p-NP法测定了活性；筛选得到了相对活性最高的脂肪分解菌，最后，通过16 s测序鉴定了富含脂肪酶菌的菌种或菌属，为进一步生物柴油的开发提供了实验基础。<br />
Lipase is the key enzyme in biodiesel production. In order to reduce the development cost of bio-diesel, lipase-rich strains were screened from the restaurant grease contamination and natural environment, and then the lipase-rich strains were mutagenized by UV and high temperature. The activity of the mutated lipolytic bacteria was identified by using Rhodamine B solid medium, and determined by P-NP method. Finally, the species or genus of lipase-rich strains were identified by 16 S sequencing, which provided an experimental basis for the further development of biodiesel.
%K 脂肪分解菌，脂肪酶，诱变，菌株筛选，p-NP法
%K Lipolytic Bacteria
%K Lipase
%K Induced Mutation
%K Strain Screening
%K p-NP Method
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=45728