%0 Journal Article
%T Effect of Excipients on Recombinant Interleukin-2 Stability in Aqueous Buffers
%A A. S. Prakasha Gowda
%A Andrew D. Schaefer
%A Terry K. Schuck
%J American Journal of Analytical Chemistry
%P 347-372
%@ 2156-8278
%D 2021
%I Scientific Research Publishing
%R 10.4236/ajac.2021.1210022
%X In order to retain structural and functional integrity, protein medicines are frequently stabilized with excipients in aqueous solutions. The goal of this investigation was to see how stable IL-2 is with excipients that are acceptable for cell therapy. We investigated the time-dependent stability of commercially available recombinant IL-2 in aqueous solutions (CTS, RPMI, PBS, and water) at different temperatures [2°C - 8°C, room temperature (20°C ¡À 2°C) and 37°C] in the presence of excipients (EDTA, methionine, histidine, and glycine) over a period of up to 30 days. To detect and quantify IL-2, reversed phase high performance liquid chromatography was employed. Electrophoresis on a sodium dodecyl sulfate polyacrylamide gel was used to assess conformational stability. We discovered that IL-2 stability was improved in aqueous solutions including excipients, and that it may have retained its biological activity and sterility in these conditions.
%K Interleukin-2
%K Excipients
%K Stability
%K RP-HPLC
%K SDS-PAGE
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=112896