%0 Journal Article
%T A Novel Label-Free Fluorescence Strategy Based on Dumbbell Probe for Sensitive Detection of DNA Ligase
%A Wenping Zhu
%A Xinlu Wang
%A Liyan Dai
%A Weijie Yang
%A Yanxia Li
%A Ruilan Liu
%J Open Access Library Journal
%V 8
%N 10
%P 1-9
%@ 2333-9721
%D 2021
%I Open Access Library
%R 10.4236/oalib.1107983
%X Based on the principle that DNA ligation reaction mediates hydrolysis of the dumbbell probe (DP), a novel label-free strategy for sensitive detection of DNA ligase was developed. DNA ligase ligated the nick on DP to generate a fully closed dumbbell DNA structure, which could prevent the hydrolysis of exonuclease, after combining with SYBR Green I (SG I), a strong fluorescent signal was obtained. In the absence of DNA ligase, DP was hydrolyzed into single nucleotides by exonuclease, resulting in a rather weak signal. The linear range of this method for detecting T4 DNA ligase is 0.00004 - 0.004 U/¦ĚL, and the detection limit is 0.00003 U/¦ĚL. The proposed strategy is sensitive, inexpensive and easy to operate, which may offer an effective tool for further applications in new drug screening.
%K Dumbbell Probe
%K DNA Ligase
%K SYBR Green I
%K DNA Ligation Reaction
%K Exonuclease
%U http://www.oalib.com/paper/6763875