%0 Journal Article
%T 人ABO血型PCR与限制性片段长度多态性的遗传分析<br>Genetic Analysis of Human ABO Blood Type by PCR and Restriction Fragment Length Polymorphism
%A 白东升
%A 康宇佳
%A 石金磊
%J Hans Journal of Biomedicine
%P 195-200
%@ 2161-8984
%D 2021
%I Hans Publishing
%R 10.12677/HJBM.2021.114025
%X 由于三个等位基因I<sup>A</sup>、I<sup>B</sup>、i的不同组合，红细胞表面带有不同的抗原，因而ABO血型分为A型、B型、AB型、O型。其中A型血个体的基因型可能是I<sup>A</sup>I<sup>A</sup>或I<sup>A</sup>i，B型血个体的基因型可能是I<sup>B</sup>I<sup>B</sup>或I<sup>B</sup>i，因此，利用血清学方法无法直接判断A或B型血个体的基因型。根据I<sup>A</sup>、I<sup>B</sup>与i基因在序列上的区别，应用PCR与限制性内切酶片段长度多态性相结合的技术，可以进行人ABO血型的基因型鉴定。本实验用PCR-RFPL法对一名A型血个体进行基因型鉴定，并用测序进行验证。结果显示，PCR-RFLP法与测序得到的结论一致，说明该方法可行性较高。<br />
Due to different combinations of alleles I<sup>A</sup>, I<sup>B</sup> and i, red blood cells carry different kinds of antigens, for which there are four blood types: A, B, AB and O in the ABO blood type system. Genotype of indi-viduals with A blood type can be I<sup>A</sup>i or I<sup>A</sup>I<sup>A</sup>, and genotype of people with B type can be I<sup>B</sup>i or I<sup>B</sup>I<sup>B</sup>. As a result, we cannot know the genotype of people with A or B blood type directly by serological meth-ods. According to the difference of sequences between I<sup>A</sup>, I<sup>B</sup> and i, we can use PCR combined re-striction fragment length polymorphism method to identify genotype of human ABO blood type. In this experiment, we analyzed genotype of one individual with A blood type by PCR-RFLP and veri-fied the result by sequencing. The result showed that the conclusion of PCR-RFLP and sequencing method is coincided, which means that PCR-RFLP is feasible.
%K PCR-RFLP，ABO血型，自主设计，开放式实验教学<br>PCR-RFPL
%K ABO Blood Type
%K Self-Design
%K Open Experimental Teaching
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=44945