%0 Journal Article
%T 16个产地北苍术苍术素含量测定及HPLC指纹图谱的建立<br>Determination of Atractylin in <i>Atractylodes lancea</i> from 16 Producing Areas and Establishment of HPLC Fingerprint
%A 杜灵均
%A 杜婧艳
%A 王建舫
%A 周志杰
%A 尹鑫
%A 田晔林
%J Hans Journal of Agricultural Sciences
%P 630-643
%@ 2164-5523
%D 2021
%I Hans Publishing
%R 10.12677/HJAS.2021.117087
%X <div style=\"text-align:justify;\">
	<span style=\"font-size:14px;\">目的：北苍术具极高的药用价值，随着野生资源过度开采濒临灭绝，人工种植北苍术刚刚起步，很多问题亟待解决。本研究旨在通过运用HPLC法对收集的16个产地的野生及人工种植的北苍术进行苍术素含量测定，筛选含量高地点的北苍术作为种源，并建立指纹图谱为北苍术质量评价提供理论依据。方法：高效液相色谱法：waters2695-2998高效液相色谱仪。色谱柱Venusil MP C18 (4.6 × 250 mm, 5 μm, 150A)，流速：1.0 mL？min<sup>？1</sup>，柱温：30℃。以甲醇为流动相A，以水为流动相B，甲醇：水 = 83:17。检测波长：254 nm。结果：河北承德地区的北苍术苍术素的含量高，尤其是滦平邓厂与北京密云交界处含量最高，为1.226%，北京房山区四马台村的最低仅0.011%。通过HPLC指纹图谱的建立，得出不同地点北苍术由于生长年限、地区和生境等因素的不同，峰图及相似度差异显著。结论：承德地区可作为北苍术种子生产基地及其成为人工种植北苍术的首选地区。<br />
Objective: <i>Atractylodes lancea</i> has extremely high medicinal value. With the over-exploitation of wild resources on the verge of extinction, artificial cultivation of <i>Atractylodes lancea</i> has just started and many problems need to be solved urgently. The purpose of this study is to determine the content of Atractylon in wild and artificially planted <i>Atractylodes lancea</i> collected from 16 producing areas by HPLC, to select <i>Atractylodes lancea</i> from high content sites as provenance, and to establish fingerprint to provide theoretical basis for quality evaluation of <i>Atractylodes lancea</i>. Methods: High performance liquid chromatography: waters2695-2998 High performance liquid chromatography. Chromatographic column Venusil MP C18 (4.6 × 250 mm, 5 μm, 150A), flow rate: 1.0 mL？min<sup>？1</sup>, column temperature: 30？C. Methanol is used as mobile phase A, water is used as mobile phase B, and methanol: water = 83:17. Detection wavelength: 254 nm. Results: The content of <i>Atractylodes lancea</i> was high in Chengde area of Hebei Province, especially at the junction of Luanping Deng Factory and Miyun of Beijing, with the highest content of 1.226%, while the lowest content was only 0.011% in Simatai Village of Fangshan District of Beijing. Through the establishment of HPLC fingerprint, it is concluded that due to the different growth years, regions, habitats and other factors of <i>Atractylodes lancea</i> in different locations, the peak figure and similarity are significantly different. Conclusion: Chengde region can be used as the seed production base of <i>Atractylodes lancea</i> and the first choice area for artificial cultivation of <i>Atractylodes lancea</i>.</span> 
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%K 北苍术，苍术素含量，高效液相色谱，指纹图谱，相似度评价<br><i>Atractylodes chinensis</i>
%K Atractylin Content
%K High Performance Liquid Chromatography
%K Fingerprints
%K Similarity Evaluation
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=43858