%0 Journal Article
%T Phytochemical Screening, Total Phenolic and Antioxidant Activity of Numerous Extracts from Leaves and Bark of <i>Bauhinia rufescens</i> Lam
%A Seid Ali Mahamat
%A Niang Lahat
%A Ayessou Nicolas Cyrille
%A Cisse Mady
%A Diop Codou Mar
%J Food and Nutrition Sciences
%P 544-556
%@ 2157-9458
%D 2021
%I Scientific Research Publishing
%R 10.4236/fns.2021.126041
%X Researches for natural antioxidants that play an important role in the
prevention of diseases linked to free radicals ha</span><span style=\"font-family:Verdana;\">ve</span><span style=\"font-family:Verdana;\"> increased in recent years. In this study,
antioxidant activity of </span><span style=\"font-family:Verdana;\">&lt;i&gt;</span><i><span style=\"font-family:Verdana;\">Bauhinia rufescens</span></i><i><span style=\"font-family:Verdana;\">&lt;/i&gt;</span></i><i><span style=\"font-family:&quot;\"> </span></i><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">Lam which is a medicinal
plant</span><i> </i><span style=\"font-family:Verdana;\">was investigated</span><i><span style=\"font-family:Verdana;\">.</span></i><span style=\"font-family:Verdana;\"> The
methanol extract, acetone extract and water extract of leaf and trunk bark had
antioxidant activity. Three methods were used: 2,2-diphenyl-1picryl-hydrazyl
(DPPH), 2,2</span></span><span style=\"font-family:Verdana;\">¡¯</span><span style=\"font-family:Verdana;\">-Azino-bis-(3-ethylbenzoth</span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:Verdana;\">iazoline-6-sulfonic acid)
(ABTS) and ferric ion reducing power (FRAP) by spectrophotometr</span><span style=\"font-family:Verdana;\">y</span><span style=\"font-family:Verdana;\">. Phytochemical screening
was carried out followed by the quantification of the total phenolic by the
Folin-Ciocalteu method. The analysis of variance (ANOVA) with the STATISTICA
7.1 software revealed significant differences (p</span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:Verdana;\">&lt;</span><span> </span><span style=\"font-family:Verdana;\">0.05). Percentage inhibition (PI) and IC</span><sub><span style=\"font-family:Verdana;\">50</span></sub><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> of the extracts were obtained using the OriginPro 8.5 software. These
tests show that the bark is as rich in total phenolic as the leaves. The DPPH
test shows that at 2.5 mg/ml, the bark and the leave have similar activity.
Bark has a percentage inhibition (PI) of 86.55% ¡À 0.026% compared to the leave
which PI is 85.6</span><span style=\"font-family:Verdana;\">3% ¡À 0</span></span><span style=\"font-family:Verdana;\">.</span><span style=\"font-family:Verdana;\">02%. As for the ABTS test, the bark extract reached its maximum activity
at 2.5 mg/ml with a PI of 99.81% ¡À 0.012% compared to the leaf extract which
has a PI of 99</span><span style=\"font-family:Verdana;\">.</span><span
%K &lt
%K i&gt
%K Bauhinia rufescens&lt
%K /i&gt
%K Lam
%K Total Phenolic
%K Antioxidant Activity
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=109969