%0 Journal Article
%T Phytochemical Composition, Anti-Inflammatory and Cytotoxic Activities of Chloroform Extract of <i>Senna crotalarioides</i> Kunth
%A Roberto Serrano-Vega
%A Salud P¨¦rez-Guti¨¦rrez
%A Francisco Alarc¨®n-Aguilar
%A Julio Almanza-P¨¦rez
%A Cuauht¨¦moc P¨¦rez-Gonz¨¢lez
%A Marco Mart¨ªn Gonz¨¢lez-Ch¨¢vez
%J American Journal of Plant Sciences
%P 887-900
%@ 2158-2750
%D 2021
%I Scientific Research Publishing
%R 10.4236/ajps.2021.126059
%X <i><span style=\"font-family:Verdana;\">Senna crotalarioides</span></i><span style=\"font-family:;\" \"=\"\"><span style=\"font-family:Verdana;\"> is used
in traditional medicine to treat inflammation. The aim of this work was to investigate
the anti-inflammatory and cytotoxic activities and the possible mechanism of
action of the chloroform extract washed with hexane of </span><i><span style=\"font-family:Verdana;\">S.</span></i> <i><span style=\"font-family:Verdana;\">crotalarioides</span></i><span style=\"font-family:Verdana;\"> (CESC). The
anti-inflammatory effect wa</span><span style=\"font-family:Verdana;\">s tested on
12-O-tetradecanoylphorbol-13-acetate (TPA)-induced </span><span style=\"font-family:Verdana;\">ear edema in mice.
The levels of TNF-</span><i><span style=\"font-family:Verdana;\">¦Á</span></i><span style=\"font-family:Verdana;\">, IL-1</span><i><span style=\"font-family:Verdana;\">¦Â</span></i><span style=\"font-family:Verdana;\">, IL-6 and IL-10 were determined in
macrophages J774A.1 stimulated by lipopolysaccharide (LPS). The cytotoxic
activity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl
tetrazolium bromide (MTT) assay against six human cancer cell lines: HeLa
(cervical cancer), SKLU-1 and A549 (lung cancer), LNCaP (prostate cancer),
SW620 (colon cancer) and MCF7 (breast cancer). The composition of the CESC was
determined by GC-MS analysis, and standardized by HPLC-ELSD with ursolic acid
as the phytochemical marker. CESC inhibited ear edema 61.45%. In chronic ear
edema, CESC diminished the inflammation by 53.77%. CESC decreased TNF-</span><i><span style=\"font-family:Verdana;\">¦Á</span></i><span style=\"font-family:Verdana;\">, IL-1</span><i><span style=\"font-family:Verdana;\">¦Â</span></i><span style=\"font-family:Verdana;\"> and IL-6 concentrations, and increased the concentration of
IL-10. The extract showed IC</span><sub><span style=\"font-family:Verdana;\">50</span></sub><span style=\"font-family:Verdana;\"> values on HeLa, SKLU-1, A549, LNCaP,
SW620 and MCF7 by 48, 21, 8.16, 6.82, 1.81, 4.06 and 12.5 ¦Ìg/mL, respectively.
The main components were ursolic acid, 1-octacosanol, stigmasterol, </span><i><span style=\"font-family:Verdana;\">¦Â</span></i><span style=\"font-family:Verdana;\">-sitosterol, 1-triacontanol, (Z, Z)
hexadec-9-enoic acid octadec-9-enyl ester. CESC might be useful for developing
a phytomedicine with anti-inflammatory and cytotoxic activities.</span></span>
%K &lt
%K i&gt
%K Senna crotalarioides&lt
%K /i&gt
%K Anti-Inflammatory
%K Ursolic Acid
%K Composition
%K Cytotoxic
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=109759