%0 Journal Article
%T 超高效液相色谱串联飞行时间质谱测定奥芬太尼及其体外代谢产物<br>Determination of Ocfentanil and Phase I Metabolites in Human Liver Microsomes by UPLC-QTOF/MS
%A 陈逸凡
%A 姜于
%A 谭鸿宇
%A 郑天
%J Advances in Analytical Chemistry
%P 68-74
%@ 2163-1565
%D 2021
%I Hans Publishing
%R 10.12677/AAC.2021.112007
%X 本文应用人肝微粒体体外温孵法模拟了奥芬太尼在人体内的代谢过程，并采用高分辨的超高效液相色谱串联三重四极杆飞行时间质谱对奥芬太尼及其在人肝微粒体中的主要代谢产物进行了分析，结果表明奥芬太尼的主要体外代谢产物由奥芬太尼的醚键去甲基生成。此结果为实际工作中筛查奥芬太尼滥用者提供了潜在的代谢标志物，但仍需在以后的工作中收集真实人体尿液样本或血液样本，比对其中的主要代谢产物，才能最终确认奥芬太尼的代谢标志物，为正确认定奥芬太尼滥用行为提供参考。<br />
UPLC-QTOF/MS was applied to the determination of ocfentanil and its major phase I metabolites in human liver microsomes. Human liver microsomes were incubated in vitro with addition of ocfentanil and one major metabolite of ocfentanil was found in human liver microsomes. O-deme- thylation was found to be the main pathway of the ocfentanil metabolism. And the O-demethylated metabolite was recommended as poisoning marker of ocfentanil in human urine and blood sample. In practice, human urine and blood sample should be collected and the main metabolite in these samples should be screened and compared in order to confirm the metabolic marker of ocfentanil.
%K 奥芬太尼，人肝微粒体，超高效液相色谱–三重四级杆飞行时间质谱法，代谢产物<br>Ocfentanil
%K Human Liver Microsome
%K UPLC-QTOF/MS
%K Metabolite
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=42267