%0 Journal Article
%T Detection of Mycoplasma bovis Infection in Cattle Mammary Tissue by Immunofluorescence and qRT-PCR Methods
%A Serdar ALTUN
%J -
%D 2019
%X Mastitis in cattle causes important economic losses in all over the world and Mycoplasma bovis is one of the important agent among pathogens shown to be responsible for this disease. The aim of this study was to determine the presence and prevalence of Mycoplasma bovis among the pathological agents implicated in mastitis by immunofluorescence staining and qRT-PCR methods in cattle mammary tissue. For this aim, 120 mammary samples with or without macroscopic lesions as this agent causes subclinical mastitis, were collected. Mastitis was diagnosed in 78 of 120 cases. Chronic mastitis cases in 56 and, acute mastitis in 22 cases were histopathologically diagnosed. Fluorescent positivity was determined 28/120 according to the results of immunofluorescence staining for all sections. Immunpositive signs of Mycoplasma bovis were commonly observed in samples with chronic mastitis. These results were confirmed by qRT-PCR. According to these results the presence of Mycoplasma bovis agent that an important threat to herd health is 23,3%
%K S£¿£¿£¿r
%K Subklinik mastitis
%K Mycoplasma bovis
%K qRT-PCR
%K £¿mmunfloresa
%U http://dergipark.org.tr/kvj/issue/43573/493259