%0 Journal Article %T Determination of Microbial Load of Injection Site in Insulin Injections %J - %D 2019 %X In this study, it was aimed to evaluate whether the use of 70% ethyl alcohol for skin antisepsis is necessary by examining the microbial load at the injection site before the injection of insulin. The study was conducted at Clinic of Endocrinology and Metabolic Diseases of an university hospital. The sample of the study consisted of 66 patients who were diagnosed with Type 1 Diabetes Mellitus (DM), were treated with insulin, had no significant dirt at the injection site, and had no risk of infection and who accepted voluntary participation in the study. Insulin injections were applied to the abdominal region and a total of two skin cultures were obtained from each patient. The first culture sample was obtained by rubbing a 5-6 cm2 area on the skin which was determined before insulin injection with a sterile swab by an experienced researcher. The area was then wiped out from inside with 70% ethyl alcohol impregnated cotton, and the alcohol was allowed to dry for 30 seconds. The patient was then given insulin injection and a second culture sample was obtained from the same site. Microbial growth was found in 95% of the patients before injection and in 66.7% of the patients after the injection. The mean Colony Forming Unit (CFU) was 60.13 ¡À 51.65 per cm2 on the injection site before injection, the mean CFU 15.98 ¡À 25.27 after using 70% ethyl alcohol and this was determined as statistically significantly higher (p = 0.000). As a result of the research; for the patients with Type 1 DM in the Clinic of Endocrinology and Metabolic Diseases, before the injection of insulin the skin antisepsis was considered to be necessary %K Cilt haz£¿rl£¿£¿£¿. Deri antisepsisi. £¿ns¨¹lin enjeksiyonu. %70¡¯lik etil alkol. %U http://dergipark.org.tr/uutfd/issue/43858/478789